[Repeated freezing of bull semen (author's transl)]. 1979

O Filseth, and T Vatn

Repeated freeze-thaw cycles have been used as a mean to predict the viability and fertility of bull semen. In order to investigate the level of fertility of bull semen that has been frozen, thawed and refrozen again, two split sample field trials were performed. 25 bulls were used in the trial, and inseminations were performed by 30 technicians. The semen was diluted to 27 million spermatozoas per dose in a skimmilk-fructose extender, and filled in the french mini-straw. The straws were coded by use of a batch number system. The one half of the straws was fixed to the freezing rampes. After freezing and transfering of the rampes to liquid nitrogen, the rampes were placed in a water-bath at + 35 degree C in 7 seconds. Immediately after thawing the straws they were transferred to a refrigidaire room at + 5 degree C, dried, remounted on the rampes and frozen again in the ordinary way. The other half of the straws were frozen according to the normal routine. The semen from the two treatments were distributed in equal numbers to the technicians who were not informed of the trial. The motility after refreezing had decreased and the percentage of intravital eosin spermatozoas after refreezing increased by 23, as an average. Fertility results were estimated as 60 days non returns after 1st inseminations. Single frozen semen: 1488 1st ins. 493 ret. 66,86 N.r.-% Refrozen semen: 1511 1st ins. 500 ret. 65,30 N.R.-% The trials indicate that further investigation should be performed to see if semen might be frozen concentrated, rediluted after thawing and refrozen for distribution to the technicians.

UI MeSH Term Description Entries
D007315 Insemination, Artificial Artificial introduction of SEMEN or SPERMATOZOA into the VAGINA to facilitate FERTILIZATION. Artificial Insemination,Eutelegenesis,Artificial Inseminations,Eutelegeneses,Inseminations, Artificial
D008297 Male Males
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D005260 Female Females
D005298 Fertility The capacity to conceive or to induce conception. It may refer to either the male or female. Fecundity,Below Replacement Fertility,Differential Fertility,Fecundability,Fertility Determinants,Fertility Incentives,Fertility Preferences,Fertility, Below Replacement,Marital Fertility,Natural Fertility,Subfecundity,World Fertility Survey,Determinant, Fertility,Determinants, Fertility,Fertility Determinant,Fertility Incentive,Fertility Preference,Fertility Survey, World,Fertility Surveys, World,Fertility, Differential,Fertility, Marital,Fertility, Natural,Preference, Fertility,Preferences, Fertility,Survey, World Fertility,Surveys, World Fertility,World Fertility Surveys
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012662 Semen Preservation The process by which semen is kept viable outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism). Frozen Semen,Sperm Preservation,Preservation, Semen,Preservation, Sperm,Semen, Frozen

Related Publications

O Filseth, and T Vatn
September 1972, DTW. Deutsche tierarztliche Wochenschrift,
O Filseth, and T Vatn
June 1975, Tijdschrift voor diergeneeskunde,
O Filseth, and T Vatn
March 1978, Zuchthygiene,
O Filseth, and T Vatn
January 1969, Deutsche tierarztliche Wochenschrift,
O Filseth, and T Vatn
September 1963, The Canadian veterinary journal = La revue veterinaire canadienne,
O Filseth, and T Vatn
May 1967, Deutsche tierarztliche Wochenschrift,
O Filseth, and T Vatn
February 2002, Journal of dairy science,
O Filseth, and T Vatn
April 1968, Deutsche tierarztliche Wochenschrift,
O Filseth, and T Vatn
July 1977, Tijdschrift voor diergeneeskunde,
Copied contents to your clipboard!