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The attachment of replicating DNA to a rapidly sedimenting nuclear structure was investigated by digestion with various nucleases. When DNA was gradually removed by DNase I, pulse label incorporated during either 1 min or during 1 hour in the presence of arabinosylcytosine, remained preferentially attached to the nuclear structure. Single strand specific digestion by nuclease S1 or staphylococcal nuclease at low concentrations caused a release of about 30% of the pulse label, without significantly affecting the attachment of randomly labelled DNA. The released material had a low sedimentation coefficient and contained most of the Okasaki fragments. The remaining pulse label was less accessible to further digestion by double strand specific nuclease activity than the bulk DNA. The results suggest that an attachment of the replication fork to the nuclear structure occurs at sites behind but close to the branch point.
P A Dijkwel, and
L H Mullenders, and
F Wanka
January 1982,
Doklady Akademii nauk SSSR,
P A Dijkwel, and
L H Mullenders, and
F Wanka
June 1984,
Biochimica et biophysica acta,
P A Dijkwel, and
L H Mullenders, and
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January 1982,
Doklady Akademii nauk SSSR,
P A Dijkwel, and
L H Mullenders, and
F Wanka
July 1993,
Journal of cell science,
P A Dijkwel, and
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F Wanka
January 2015,
Plant biology (Stuttgart, Germany),
P A Dijkwel, and
L H Mullenders, and
F Wanka
January 1991,
Annals of the New York Academy of Sciences,
P A Dijkwel, and
L H Mullenders, and
F Wanka
July 1982,
Biochimica et biophysica acta,
P A Dijkwel, and
L H Mullenders, and
F Wanka
February 1991,
Experimental cell research,
P A Dijkwel, and
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F Wanka
November 1981,
Cell,
P A Dijkwel, and
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F Wanka
February 1974,
Tsitologiia,
