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Cultured skin fibroblasts from subjects with cystic fibrosis exhibited normal population doubling times in early passages. After about 13 cumulative population doublings, cystic fibrosis lines doubled more slowly than controls and ceased doubling after about 19 weekly passages. Control lines continued doubling for 27 passages. The premature senescence noted in cells from subjects with cystic fibrosis reconciles controversial observations of cell doubling reported in the literature. Data presented here demonstrate that experiments with cystic fibrosis cells in late passage may generate misleading results since differences from control lines may be ascribed to generalized senile changes rather than to specific results of the cystic fibrosis genotype.
B L Shapiro, and
L F Lam, and
L H Fast
December 1981,
Biochemical medicine,
B L Shapiro, and
L F Lam, and
L H Fast
July 1974,
Irish journal of medical science,
B L Shapiro, and
L F Lam, and
L H Fast
May 1987,
Proceedings of the National Academy of Sciences of the United States of America,
B L Shapiro, and
L F Lam, and
L H Fast
July 1981,
Journal of submicroscopic cytology,
B L Shapiro, and
L F Lam, and
L H Fast
January 1976,
Texas reports on biology and medicine,
B L Shapiro, and
L F Lam, and
L H Fast
June 1974,
Biochemical and biophysical research communications,
B L Shapiro, and
L F Lam, and
L H Fast
January 1982,
Scanning electron microscopy,
B L Shapiro, and
L F Lam, and
L H Fast
June 1971,
Pediatrics,
B L Shapiro, and
L F Lam, and
L H Fast
January 1977,
Clinica chimica acta; international journal of clinical chemistry,
B L Shapiro, and
L F Lam, and
L H Fast
December 1971,
Journal of medical genetics,
