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An improved method of purification of DNA methylase from Krebs II ascites cells is reported. The enzyme sediments at 8.3 S on glycerol-gradients and a major band on SDS polyacrylamide gel electrophoresis has a molecular weight of 184 000. Aggregation occurs at low salt and this may interfere with enzymic activity. The preferred double stranded DNA substrate is that rendered partially unmethylated by an in vitro repair mechanism or by isolation from methionine starved cells. Methylation of native partially methylated DNA is favoured under conditions of low salt and high temperature; conditions which encourage 'breathing' of the DNA. Methylation of native, unmethylated DNA also involves breathing but results in formation of a salt resistant tight binding complex between the enzyme and the DNA.
R L Adams, and
E L McKay, and
L M Craig, and
R H Burdon
January 1981,
Carcinogenesis,
R L Adams, and
E L McKay, and
L M Craig, and
R H Burdon
November 1983,
FEBS letters,
R L Adams, and
E L McKay, and
L M Craig, and
R H Burdon
January 1985,
Biochemical and biophysical research communications,
R L Adams, and
E L McKay, and
L M Craig, and
R H Burdon
May 1972,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
R L Adams, and
E L McKay, and
L M Craig, and
R H Burdon
June 1993,
Biochemical and biophysical research communications,
R L Adams, and
E L McKay, and
L M Craig, and
R H Burdon
February 1981,
Nucleic acids research,
R L Adams, and
E L McKay, and
L M Craig, and
R H Burdon
January 1984,
Current topics in microbiology and immunology,
R L Adams, and
E L McKay, and
L M Craig, and
R H Burdon
January 1989,
Cell biophysics,
R L Adams, and
E L McKay, and
L M Craig, and
R H Burdon
January 1980,
Doklady Akademii nauk SSSR,
R L Adams, and
E L McKay, and
L M Craig, and
R H Burdon
November 1976,
Biokhimiia (Moscow, Russia),
