-transparent.png){kind=logo}
The hydroxysteroid dehydrogenases: beta-HSDH, 20 beta-HSDH, and 3 alpha-HSDH, were immobilized on CNBr-activated Sepharose. The effect of various immobilization conditions on the activity recovery and stability were examined. The presence of cofactor during the immobilization reaction increased the activity recovery (40--60% of the total) and also led to materials highly stable in the presence of organic solvents. For example, beta-HSDH maintained 60% of its original activity two months after continuous use in the water--ethyl-acetate system. Kinetic experiments showed that the increase of the apparent Km values is poor and demonstrated that the organic solvent behaves as a weak inhibitor (ki greater than 0.2M) for the substrate. The immobilized enzymes lyophilized in the presence of sucrose had full activity restored even after several months storage at room temperature. Immobilized hydroxysteroid dehydrogenases were shown to be suitable for preparative transformation of steroids in water--organic solvent systems.
G Carrea, and
F Colombi, and
G Mazzola, and
P Cremonesi, and
E Antonini
January 1988,
Annals of the New York Academy of Sciences,
G Carrea, and
F Colombi, and
G Mazzola, and
P Cremonesi, and
E Antonini
February 1984,
Analytical biochemistry,
G Carrea, and
F Colombi, and
G Mazzola, and
P Cremonesi, and
E Antonini
January 1983,
Prikladnaia biokhimiia i mikrobiologiia,
G Carrea, and
F Colombi, and
G Mazzola, and
P Cremonesi, and
E Antonini
March 1968,
Journal of the American Chemical Society,
G Carrea, and
F Colombi, and
G Mazzola, and
P Cremonesi, and
E Antonini
December 1948,
The Journal of physical and colloid chemistry,
G Carrea, and
F Colombi, and
G Mazzola, and
P Cremonesi, and
E Antonini
March 1991,
World journal of microbiology & biotechnology,
G Carrea, and
F Colombi, and
G Mazzola, and
P Cremonesi, and
E Antonini
July 1968,
Biochemistry,
G Carrea, and
F Colombi, and
G Mazzola, and
P Cremonesi, and
E Antonini
April 1949,
Journal of the American Chemical Society,
G Carrea, and
F Colombi, and
G Mazzola, and
P Cremonesi, and
E Antonini
January 1990,
Annals of the New York Academy of Sciences,
G Carrea, and
F Colombi, and
G Mazzola, and
P Cremonesi, and
E Antonini
July 1982,
Steroids,
