Biomaterial Database

Improved rapid plate method for the isolation of bacteriophages from lysogenic bacteria. 1974

J T Parisi, and H W Talbot

A modification of a recently reported rapid plate method for the isolation of bacteriophages from lysogenic bacteria is described. The velveteen replica plate technique was used for inoculation of mitomycin C-induced colonies onto agar plates, and tetrazolium chloride was used to enhance detection of phage activity on replicated indicator plates.

UI	MeSH Term	Description	Entries
D008242	Lysogeny	The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.	Integration, Prophage,Prophage Integration,Integrations, Prophage,Prophage Integrations
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D008937	Mitomycins	A group of methylazirinopyrroloindolediones obtained from certain Streptomyces strains. They are very toxic antibiotics used as ANTINEOPLASTIC AGENTS in some solid tumors. PORFIROMYCIN and MITOMYCIN are the most useful members of the group.
D000362	Agar	A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
D013204	Staphylococcus Phages	Viruses whose host is Staphylococcus.	Staphylococcal Phages,Staphylococcal Bacteriophage,Staphylococcal Bacteriophages,Staphylococcus Phage,Bacteriophage, Staphylococcal,Bacteriophages, Staphylococcal,Phage, Staphylococcal,Phage, Staphylococcus,Phages, Staphylococcal,Phages, Staphylococcus,Staphylococcal Phage
D013210	Staphylococcus	A genus of gram-positive, facultatively anaerobic, coccoid bacteria. Its organisms occur singly, in pairs, and in tetrads and characteristically divide in more than one plane to form irregular clusters. Natural populations of Staphylococcus are found on the skin and mucous membranes of warm-blooded animals. Some species are opportunistic pathogens of humans and animals.
D013778	Tetrazolium Salts	Quaternary salts derived from tetrazoles. They are used in tests to distinguish between reducing sugars and simple aldehydes, for detection of dehydrogenase in tissues, cells, and bacteria, for determination of corticosteroids, and in color photography. (From Mall's Dictionary of Chemistry, 5th ed, p455)	Tetrazolium Salt,Salt, Tetrazolium,Salts, Tetrazolium
D014779	Virus Replication	The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.	Viral Replication,Replication, Viral,Replication, Virus,Replications, Viral,Replications, Virus,Viral Replications,Virus Replications

Related Publications

J T Parisi, and H W Talbot

Rapid plate method for the isolation of lysogenic bacteriophages.

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J T Parisi, and H W Talbot

Rapid method for the isolation of bacteriophages from lysogens.

November 1988, Diagnostic microbiology and infectious disease,

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[Isolation of bacteriophages from lysogenic Nocardia asteroides strains].

March 1972, Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie,

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[Secondary bacteriophages infections of lysogenic bacteria].

January 1953, Annales de l'Institut Pasteur,

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The isolation of desoxyribonucleic acid from bacteriophages by an improved method.

October 1954, The Journal of biological chemistry,

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[Induction of the production of bacteriophages in lysogenic bacteria].

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Suppression of the multiplication of heterologous bacteriophages in lysogenic bacteria.

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