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Kinetics of transport and metabolism of bromosulfophthalein have been studied in isolated liver cells in a dose-dependent manner obtaining the following results. The disposition of bromosulfophthalein in suspensions of isolated liver cells is similar to the turnover in the whole liver. The initial maximal rate of uptake of bromosulfophthalein is 2--3 times faster than intracellular conjugation with glutathione. Conjugation proceeds to an equilibrium between intracellular substrate (bromosulfophthalein) and product (bromosulfophthalein-glutathione conjugate) which are both transiently accumulated in the cell. Formation of bromosulfophthalein-glutathione is accompanied by an equimolar decrease of glutathione. The bromosulfophthalein-glutathione conjugate is slowly released from the cells in an energy-dependent and saturable transport process. The maximal velocity of excretion amounts to only 6% of the maximal velocity of uptake and to 20% of the maximal velocity of conjugation. Excretion, therefore, represents the slowest step in the overall turnover.
L R Schwarz, and
K H Summer, and
M Schwenk
April 1976,
European journal of biochemistry,
L R Schwarz, and
K H Summer, and
M Schwenk
January 1984,
Hepatology (Baltimore, Md.),
L R Schwarz, and
K H Summer, and
M Schwenk
September 1983,
Toxicology,
L R Schwarz, and
K H Summer, and
M Schwenk
October 1969,
The Biochemical journal,
L R Schwarz, and
K H Summer, and
M Schwenk
January 1975,
Advances in experimental medicine and biology,
L R Schwarz, and
K H Summer, and
M Schwenk
March 1978,
The Biochemical journal,
L R Schwarz, and
K H Summer, and
M Schwenk
October 2002,
Toxicological sciences : an official journal of the Society of Toxicology,
L R Schwarz, and
K H Summer, and
M Schwenk
May 1982,
Biochemical pharmacology,
L R Schwarz, and
K H Summer, and
M Schwenk
January 1995,
Anticancer research,
L R Schwarz, and
K H Summer, and
M Schwenk
September 1970,
Biochimica et biophysica acta,
