Biomaterial Database

Histochemical demonstration of alkaline phosphatase in human large intestine, normal and diseased. 1979

L Bell, and L Williams

Alkaline phosphatase activity has been investigated by histochemical methods in normal and diseased human large intestine. The tissues were constantly maintained at 4 degrees C or below. Specimens were either frozen in liquid nitrogen, freeze-dried and embedded in glycol methacrylate for sectioning at 2 mu, or, fixed in ice-cold formol-calcium for frozen sectioning at 10 mu. The simultaneous coupling azo dye method using the substrates sodium alpha-naphthyl phosphate and Naphthol AS-BI phosphate, resulted in the demonstration of alkaline phosphatase activity in the surface epithelial cells, and the middle and upper crypts, of normal and transitional mucosa.

UI	MeSH Term	Description	Entries
D007413	Intestinal Mucosa	Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.	Intestinal Epithelium,Intestinal Glands,Epithelium, Intestinal,Gland, Intestinal,Glands, Intestinal,Intestinal Gland,Mucosa, Intestinal
D007420	Intestine, Large	A segment of the LOWER GASTROINTESTINAL TRACT that includes the CECUM; the COLON; and the RECTUM.	Large Intestine
D002430	Cecal Neoplasms	Tumors or cancer of the CECUM.	Cancer of Cecum,Cecal Cancer,Cancer of the Cecum,Cancer, Cecal,Neoplasms, Cecal,Cecal Neoplasm,Neoplasm, Cecal
D004848	Epithelium	The layers of EPITHELIAL CELLS which cover the inner and outer surfaces of the cutaneous, mucus, and serous tissues and glands of the body.	Mesothelium,Epithelial Tissue,Mesothelial Tissue,Epithelial Tissues,Mesothelial Tissues,Tissue, Epithelial,Tissue, Mesothelial,Tissues, Epithelial,Tissues, Mesothelial
D006651	Histocytochemistry	Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.	Cytochemistry
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000469	Alkaline Phosphatase	An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.
D012811	Sigmoid Neoplasms	Tumors or cancer of the SIGMOID COLON.	Cancer of Sigmoid,Sigmoid Cancer,Cancer of the Sigmoid,Neoplasms, Sigmoid,Sigmoid Colon Cancer,Sigmoid Colon Neoplasms,Sigmoidal Cancer,Cancer, Sigmoid,Cancer, Sigmoid Colon,Colon Cancer, Sigmoid,Colon Neoplasms, Sigmoid,Neoplasm, Sigmoid,Neoplasm, Sigmoid Colon,Neoplasms, Sigmoid Colon,Sigmoid Colon Neoplasm,Sigmoid Neoplasm