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Specific cytosol receptors for 1,25-dihydroxyvitamin D3 in human intestine. 1979

W R Wecksler, and R S Mason, and A W Norman

Human intestine is shown to contain a specific, high affinity cytosol receptor or binding protein for 1,25-(OH)2-vitamin D3 [1,25 (OH)2D3]. This receptor is a protein which sediments at 3.5S on sucrose gradients containing 0.3 M KCL. The receptor binds 1,25 (OH)2D3 with a Kd of approximately 2 x 10(-10) M at 4 degrees C. Competition binding studies using structural analogs of 1,25 (OH)2D3 indicate that the relative specificity of the receptor is 1,25 (OH)2D3 greater than 1,24R,25(OH)3D3 greater than greater than 25OHD3 1 OHD3 greater than 24R,25(OH)2D3.

UI MeSH Term Description Entries
D007583 Jejunum The middle portion of the SMALL INTESTINE, between DUODENUM and ILEUM. It represents about 2/5 of the remaining portion of the small intestine below duodenum. Jejunums
D007700 Kinetics The rate dynamics in chemical or physical systems.
D011955 Receptors, Drug Proteins that bind specific drugs with high affinity and trigger intracellular changes influencing the behavior of cells. Drug receptors are generally thought to be receptors for some endogenous substance not otherwise specified. Drug Receptors,Drug Receptor,Receptor, Drug
D003600 Cytosol Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components. Cytosols
D004100 Dihydroxycholecalciferols Cholecalciferols substituted with two hydroxy groups in any position. Dihydroxyvitamins D
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006887 Hydroxycholecalciferols Hydroxy analogs of vitamin D 3; (CHOLECALCIFEROL); including CALCIFEDIOL; CALCITRIOL; and 24,25-DIHYDROXYVITAMIN D 3. Hydroxyvitamins D,Hydroxycholecalciferol
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D001667 Binding, Competitive The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements. Competitive Binding

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