Biomaterial Database

In vitro studies of fatty acid metabolism in vitamin B6 deficient rats. 1979

P E Dussault, and M Lepage

The oxidation of [1-14C]palmitate, [1-14C]linoleate, and [1-14C]arachidonate as well as their incorporation into lipids was investigated in vitamin B6 deficient rats using liver slices and isolated mitochondria. In experiments on fatty acid oxidation in liver slices, we observed a decrease in the production of 14CO2 from radioactive palmitate and linoleate, but not from arachidonate. In the case of mitochondria, we detected no difference between the deficient rats and the controls with linoleats; however, there was an increase in the oxidation of palmitate in the deficient rats as compared to the controls. Experiments on the incorporation of radioactivity into lipids showed an increase in radioactivity from [1-14C]palmitate and [1-14C]linoleate in the triglyceride, phospholipid and cholesterol fractions of deficient rats. In the case of [1-14C]arachidonate, no difference was observed between the two groups of rats. These experiments showed that major changes occurred in metabolism of palmitate and linoleate and strongly suggested that the decrease in the arachidonate content previously observed in rat tissues could not be explained by alteration in the metabolism of this fatty acid.

UI	MeSH Term	Description	Entries
D008041	Linoleic Acids	Eighteen-carbon essential fatty acids that contain two double bonds.	Acids, Linoleic
D008055	Lipids	A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)	Lipid
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008297	Male		Males
D010169	Palmitic Acids	A group of 16-carbon fatty acids that contain no double bonds.	Acids, Palmitic
D010743	Phospholipids	Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.	Phosphatides,Phospholipid
D002784	Cholesterol	The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.	Epicholesterol
D005230	Fatty Acids, Nonesterified	FATTY ACIDS found in the plasma that are complexed with SERUM ALBUMIN for transport. These fatty acids are not in glycerol ester form.	Fatty Acids, Free,Free Fatty Acid,Free Fatty Acids,NEFA,Acid, Free Fatty,Acids, Free Fatty,Acids, Nonesterified Fatty,Fatty Acid, Free,Nonesterified Fatty Acids
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001095	Arachidonic Acids		Eicosatetraenoic Acids,Acids, Arachidonic,Acids, Eicosatetraenoic