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A comparison was made between a macrotechnique in tubes and a microtechnique in plastic plates for complement-fixation tests, using strains of three subtypes of the Asia 1 type of foot-and-mouth disease (FMD) virus. The results obtained with these techniques were found to be comparable and delineated the antigenic relationships of the three strains employed. The microtechnique was considered to be both economical with reagents and capable of similar accuracy and reproducibility to that of the standard method in tubes. It was concluded that the antigenic analysis of subtype strains of FMD virus can be conveniently carried out by the use of the microtechnique as described.
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