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A sensitive (sensitivity 2.2 X 10(-9) mol/l) and specific (practically no cross-reaction with circulating folates) radioimmunoassay for the determination of methotrexate concentrations in biological fluids in described and compared with a commercial competitive protein binding assay. Antiserum with high titer was produced in rabbits immunized with MTX-human serum albumin conjugate. Fitness for use in pharmacokinetic drug level determinations was shown in three patients, who received both low doses and high dose therapy combined with citrovorum factor rescue. An excellent correlation was found between plasma and urine MTX concentrations obtained by RIA and competitive protein binding assay. A two-compartment pharmacokinetic model was found adequately describing the serum decay curves, but there was a great interindividual variability in the calculated pharmacokinetic parameters.
R Virtanen, and
E Iisalo, and
M Parvinen, and
E Nordman
August 1975,
Cancer research,
R Virtanen, and
E Iisalo, and
M Parvinen, and
E Nordman
December 1974,
Clinica chimica acta; international journal of clinical chemistry,
R Virtanen, and
E Iisalo, and
M Parvinen, and
E Nordman
June 1976,
Clinical chemistry,
R Virtanen, and
E Iisalo, and
M Parvinen, and
E Nordman
July 1977,
Cancer treatment reports,
R Virtanen, and
E Iisalo, and
M Parvinen, and
E Nordman
June 1959,
Nature,
R Virtanen, and
E Iisalo, and
M Parvinen, and
E Nordman
July 1985,
Toxicology and applied pharmacology,
R Virtanen, and
E Iisalo, and
M Parvinen, and
E Nordman
May 1976,
NIDA research monograph,
R Virtanen, and
E Iisalo, and
M Parvinen, and
E Nordman
January 1977,
Acta obstetricia et gynecologica Scandinavica. Supplement,
R Virtanen, and
E Iisalo, and
M Parvinen, and
E Nordman
April 1977,
Journal of pharmaceutical sciences,
R Virtanen, and
E Iisalo, and
M Parvinen, and
E Nordman
January 1975,
Journal of steroid biochemistry,
