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The major apoprotein(s) from human plasma low density lipoproteins was isolated and compared with a major protein fraction (fraction I) from very low density lipoproteins (VLDL). Fraction I had been previously found to comprise approximately 40% of the total protein of VLDL. Fraction I from VLDL and apoLDL from normal subjects were indistinguishable in amino acid compositions and circular dichroic spectra. They yielded indistinguishable displacement curves of LDL-(125)I by radioimmunoassay and formed immunoprecipitin lines of complete identity. Fraction I from VLDL of normal subjects was compared with the fraction isolated from patients with familial types II, III, IV, and V hyperlipoproteinemia. There were no detectable differences between any of these fractions in amino acid compositions, circular dichroic spectra, and immunochemical properties. It was, therefore, concluded that short of peptide mapping or determination of amino acid sequence, fraction I from VLDL of each subject with familial hyperlipoproteinemia appears to be identical with fraction I and apoLDL from normal individuals.A new convenient method of preparation of soluble apoLDL, modified from a procedure previously described from this laboratory, is presented.
A M Gotto, and
W V Brown, and
R I Levy, and
M E Birnbaumer, and
D S Fredrickson
June 1981,
The Journal of laboratory and clinical medicine,
A M Gotto, and
W V Brown, and
R I Levy, and
M E Birnbaumer, and
D S Fredrickson
December 1982,
The Tohoku journal of experimental medicine,
A M Gotto, and
W V Brown, and
R I Levy, and
M E Birnbaumer, and
D S Fredrickson
August 1988,
The Journal of clinical investigation,
A M Gotto, and
W V Brown, and
R I Levy, and
M E Birnbaumer, and
D S Fredrickson
June 1982,
The Journal of clinical endocrinology and metabolism,
A M Gotto, and
W V Brown, and
R I Levy, and
M E Birnbaumer, and
D S Fredrickson
January 1977,
The Journal of biological chemistry,
A M Gotto, and
W V Brown, and
R I Levy, and
M E Birnbaumer, and
D S Fredrickson
January 1982,
Arteriosclerosis (Dallas, Tex.),
A M Gotto, and
W V Brown, and
R I Levy, and
M E Birnbaumer, and
D S Fredrickson
November 1980,
Biochemistry,
A M Gotto, and
W V Brown, and
R I Levy, and
M E Birnbaumer, and
D S Fredrickson
December 1975,
The Journal of clinical investigation,
A M Gotto, and
W V Brown, and
R I Levy, and
M E Birnbaumer, and
D S Fredrickson
March 1974,
The Journal of biological chemistry,
A M Gotto, and
W V Brown, and
R I Levy, and
M E Birnbaumer, and
D S Fredrickson
June 1982,
Atherosclerosis,
