Biomaterial Database

A micro-passive hemagglutination test for the rapid detection of antibodies to infectious bovine rhinotracheitis virus. 1971

V E Vengris, and C J Maré

Serum antibodies to infectious bovine rhinotracheitis virus are most commonly detected using the serum-virus-neutralization test, a time-consuming and expensive procedure. A passive hemagglutination test was developed for the rapid detection of antibodies using the Microtiter System. Two cloned strains of the virus were used in the investigation, namely the BF strain and the Los Angeles strain. Erythrocytes from several species were used. The best results were obtained using the Los Angeles strain of virus adsorbed to sheep cells treated with formalin and tannic acid. Sera from field cases of the disease as well as hyperimmune bovine and rabbit sera were tested using both the micro-passive hemagglutination test and the standard plaque-neutralization procedures. Comparable titers were obtained in the two tests. The micro-passive hemagglutination test is rapid, accurate and economical.

UI	MeSH Term	Description	Entries
D007242	Herpesvirus 1, Bovine	A species of VARICELLOVIRUS that causes INFECTIOUS BOVINE RHINOTRACHEITIS and other associated syndromes in CATTLE.	IBR-IPV Virus,Infectious Bovine Rhinotracheitis Virus,Infectious Pustular Vulvovaginitis Virus,Bovine Herpesvirus 1,Bovine Rhinotracheitis Virus, Infectious,Herpesvirus 1 (alpha), Bovine,Pustular Vulvovaginitis Virus, Infectious,IBR IPV Virus,IBR-IPV Viruses,Virus, IBR-IPV,Viruses, IBR-IPV
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D009500	Neutralization Tests	The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).	Neutralization Test,Test, Neutralization,Tests, Neutralization
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002418	Cattle Diseases	Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus.	Bovine Diseases,Bovine Disease,Cattle Disease,Disease, Bovine,Disease, Cattle,Diseases, Bovine,Diseases, Cattle
D002999	Clone Cells	A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)	Clones,Cell, Clone,Cells, Clone,Clone,Clone Cell
D006386	Hemagglutination Tests	Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)	Hemagglutination Test,Test, Hemagglutination,Tests, Hemagglutination
D006387	Hemagglutination, Viral	Agglutination of ERYTHROCYTES by a virus.	Hemagglutinations, Viral,Viral Hemagglutination,Viral Hemagglutinations
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000906	Antibodies	Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).