Biomaterial Database

Heat production and fluorescence changes of toad sartorius muscle during aerobic recovery after a short tetanus. 1972

A Godfraind-de Becker

1. The time course of the aerobic recovery following a 0.5 sec tetanus at 20 degrees C of the sartorius muscle of the toad Bufo bufo, equilibrated in bicarbonate-CO(2) Ringer solution, has been followed by recording simultaneously the heat production and the fluorescence excited by ultra-violet light at 366 nm.2. The fluorescence light emitted in these conditions in the region of 450 nm monitors the state of oxidation-reduction of the nicotinamideadenine dinucleotides (NAD(+)-NADH). After a short tetanus, the cycle evoked consists of an initial increase of the fluorescence (reduction of NAD(+)) followed by a long lasting phase of decreased light emission. This includes an early period of oxidation of NADH succeeded by a slow reduction of the NAD(+) formed in excess over the resting state. After iodoacetate, the initial reduction is suppressed.3. The time course of both fluorescence and heat production may be analysed into a rapid and a slow component by a double exponential model.4. The time courses of the aerobic recovery heat and of the fluorescence changes are similar after five minutes, but differ in their fast components. IAA significantly increases the rate constants of the fast terms of both monitors.5. The slow component is mainly related to aerobic processes while the fast one is due to both oxidative and glycolytic reactions occurring simultaneously.

UI	MeSH Term	Description	Entries
D007461	Iodoacetates	Iodinated derivatives of acetic acid. Iodoacetates are commonly used as alkylating sulfhydryl reagents and enzyme inhibitors in biochemical research.	Iodoacetic Acids,Acids, Iodoacetic
D009119	Muscle Contraction	A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments.	Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D009132	Muscles	Contractile tissue that produces movement in animals.	Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D009243	NAD	A coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). (Dorland, 27th ed)	Coenzyme I,DPN,Diphosphopyridine Nucleotide,Nadide,Nicotinamide-Adenine Dinucleotide,Dihydronicotinamide Adenine Dinucleotide,NADH,Adenine Dinucleotide, Dihydronicotinamide,Dinucleotide, Dihydronicotinamide Adenine,Dinucleotide, Nicotinamide-Adenine,Nicotinamide Adenine Dinucleotide,Nucleotide, Diphosphopyridine
D010101	Oxygen Consumption	The rate at which oxygen is used by a tissue; microliters of oxygen STPD used per milligram of tissue per hour; the rate at which oxygen enters the blood from alveolar gas, equal in the steady state to the consumption of oxygen by tissue metabolism throughout the body. (Stedman, 25th ed, p346)	Consumption, Oxygen,Consumptions, Oxygen,Oxygen Consumptions
D002023	Bufo bufo	A species of the true toads, Bufonidae, widely distributed in the United States and Europe.	Toad, Common,Common Toad,Common Toads,Toads, Common
D005453	Fluorescence	The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
D006003	Glycogen
D006358	Hot Temperature	Presence of warmth or heat or a temperature notably higher than an accustomed norm.	Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D000332	Aerobiosis	Life or metabolic reactions occurring in an environment containing oxygen.	Aerobioses