BIOMAT Database Logo BIOMAT Database Logo

Effect of adrenaline on 32 P incorporation into rat fat-cell phospholipids. 1972

J M Stein, and C N Hales

1. The phospholipid composition of fat-cells prepared from rat epididymal fat-pad was determined. 2. The incorporation of [(32)P]P(i) into the phospholipids of fat-cells incubated in glucose-free medium and the effect of adrenaline and of alpha- and beta-adrenergic blocking agents, were studied. 3. Incorporation of [(32)P]P(i) into fat-cell phospholipid increased with time; incubation with adrenaline resulted in increased incorporation that was related to the concentration of adrenaline. 4. The pattern of incorporation of [(32)P]P(i) into the individual phospholipids of fat-cells after incubation for 1h was determined; adrenaline (5.4mum) resulted in increased incorporation into phosphatidylcholine. 5. Incubation of fat-cells with propranolol (34mum) and adrenaline (5.4mum) resulted in abolition of adrenaline-stimulated lipolysis; there was a decrease in the specific radioactivity of phosphatidylcholine and an increase in the specific radioactivity of phosphatidylethanolamine, phosphatidic acid, phosphatidylinositol and cardiolipin compared with cells incubated with adrenaline alone. 6. Incubation of fat-cells with phenoxybenzamine (0.1mm) and adrenaline (5.4mum) resulted in stimulation of lipolysis, and in diminished specific radioactivities of phosphatidylcholine, phosphatidic acid, phosphatidylinositol, phosphatidylglycerol and choline plasmalogen compared with cells stimulated with adrenaline alone.

UI MeSH Term Description Entries
D008297 Male Males
D010643 Phenoxybenzamine An alpha-adrenergic antagonist with long duration of action. It has been used to treat hypertension and as a peripheral vasodilator. Dibenylene,Dibenyline,Dibenziran,Dibenzylin,Dibenzyline,Dibenzyran,Phenoxybenzamine Hydrochloride,Hydrochloride, Phenoxybenzamine
D010646 Phentolamine A nonselective alpha-adrenergic antagonist. It is used in the treatment of hypertension and hypertensive emergencies, pheochromocytoma, vasospasm of RAYNAUD DISEASE and frostbite, clonidine withdrawal syndrome, impotence, and peripheral vascular disease. Fentolamin,Phentolamine Mesilate,Phentolamine Mesylate,Phentolamine Methanesulfonate,Phentolamine Mono-hydrochloride,Regitine,Regityn,Rogitine,Z-Max,Mesilate, Phentolamine,Mesylate, Phentolamine,Methanesulfonate, Phentolamine,Mono-hydrochloride, Phentolamine,Phentolamine Mono hydrochloride
D010713 Phosphatidylcholines Derivatives of PHOSPHATIDIC ACIDS in which the phosphoric acid is bound in ester linkage to a CHOLINE moiety. Choline Phosphoglycerides,Choline Glycerophospholipids,Phosphatidyl Choline,Phosphatidyl Cholines,Phosphatidylcholine,Choline, Phosphatidyl,Cholines, Phosphatidyl,Glycerophospholipids, Choline,Phosphoglycerides, Choline
D010714 Phosphatidylethanolamines Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to an ethanolamine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and ethanolamine and 2 moles of fatty acids. Cephalin,Cephalins,Ethanolamine Phosphoglyceride,Ethanolamine Phosphoglycerides,Ethanolamineglycerophospholipids,Phosphoglyceride, Ethanolamine,Phosphoglycerides, Ethanolamine
D010716 Phosphatidylinositols Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to the hexahydroxy alcohol, myo-inositol. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid, myo-inositol, and 2 moles of fatty acids. Inositide Phospholipid,Inositol Phosphoglyceride,Inositol Phosphoglycerides,Inositol Phospholipid,Phosphoinositide,Phosphoinositides,PtdIns,Inositide Phospholipids,Inositol Phospholipids,Phosphatidyl Inositol,Phosphatidylinositol,Inositol, Phosphatidyl,Phosphoglyceride, Inositol,Phosphoglycerides, Inositol,Phospholipid, Inositide,Phospholipid, Inositol,Phospholipids, Inositide,Phospholipids, Inositol
D010743 Phospholipids Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system. Phosphatides,Phospholipid
D010758 Phosphorus A non-metal element that has the atomic symbol P, atomic number 15, and atomic weight 31. It is an essential element that takes part in a broad variety of biochemical reactions. Black Phosphorus,Phosphorus-31,Red Phosphorus,White Phosphorus,Yellow Phosphorus,Phosphorus 31,Phosphorus, Black,Phosphorus, Red,Phosphorus, White,Phosphorus, Yellow
D010759 Phosphorus Isotopes Stable phosphorus atoms that have the same atomic number as the element phosphorus, but differ in atomic weight. P-31 is a stable phosphorus isotope. Isotopes, Phosphorus
D011433 Propranolol A widely used non-cardioselective beta-adrenergic antagonist. Propranolol has been used for MYOCARDIAL INFARCTION; ARRHYTHMIA; ANGINA PECTORIS; HYPERTENSION; HYPERTHYROIDISM; MIGRAINE; PHEOCHROMOCYTOMA; and ANXIETY but adverse effects instigate replacement by newer drugs. Dexpropranolol,AY-20694,Anaprilin,Anapriline,Avlocardyl,Betadren,Dociton,Inderal,Obsidan,Obzidan,Propanolol,Propranolol Hydrochloride,Rexigen,AY 20694,AY20694,Hydrochloride, Propranolol

Related Publications

J M Stein, and C N Hales
The effect of noradrenaline on the incorporation of 32 P into brain phospholipids.
April 1970, Biochemical pharmacology,
J M Stein, and C N Hales
[Incorporation of P-32 into phospholipids. 3. Effect of acute and chronic administration of glucose on the incorporation of P-32 into pancreatic phospholipids and of ethanol into phospholipids of the liver].
September 1968, Revista medica de Chile,
J M Stein, and C N Hales
In vivo incorporation of P-32 into chicken serum phospholipids.
June 1965, Canadian journal of biochemistry,
J M Stein, and C N Hales
Pattern of incorporation of 32 P into the phospholipids of the rat tapeworm Hymenolepis diminuta.
November 1971, Canadian journal of biochemistry,
J M Stein, and C N Hales
Effect of acute hypobaric hypoxia on 32-P incorporation into phospholipids of alveolar surfactant, lung, liver and plasma of rat.
January 1975, Environmental physiology & biochemistry,
J M Stein, and C N Hales
Effects of L-thyroxine on incorporation of (32)P into phospholipids of freshwater eels.
March 2001, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,
J M Stein, and C N Hales
Effect of TSH, acetylcholine, epinephrine, serotonin and synkavite on 32-P incorporation into phospholipids in dog-thyroid slices.
June 1966, Biochimica et biophysica acta,
J M Stein, and C N Hales
[On the incorporation of P-32 into inositol phosphatides of rat brain].
January 1961, Biochemische Zeitschrift,
J M Stein, and C N Hales
Incorporation of 32 P into the DNA of rat lymphocytes.
August 1958, Experimental cell research,
J M Stein, and C N Hales
[Metabolism of phospholipids of the aorta. Incorporation of (32-p) orthophosphate into the phospholipids of the aorta of the rat in vivo].
January 1974, Atherosclerosis,
Copied contents to your clipboard!