Biomaterial Database

[Temperature characteristics of an object during the process of cryofixation by the "freeze--etching" technic]. 1979

E M Belavtseva, and I I Chemeris

When using low temperature methods for preparing water-containing objects in electron microscopy temperature characteristics of different objects was studied during freezing and defreezing depending on different preparation conditions. Temperature characteristics of the object was estimated by means of a storage oscillograph with a thermocouple connected through a transistor amplifier. The thermograms permit one to select preparation conditions of the object eliminating artefacts.

UI	MeSH Term	Description	Entries
D008875	Middle Aged	An adult aged 45 - 64 years.	Middle Age
D009132	Muscles	Contractile tissue that produces movement in animals.	Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D005613	Freeze Etching	A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces.	Etching, Freeze
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013696	Temperature	The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.	Temperatures