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A mutant line of 3T3 (mouse fibroblast) cells with temperature-sensitive cyclic AMP (cAMP) metabolism was isolated by selecting for low substratum adhesiveness after a change in temperature. Although the mutant is identical in behavior to the parent cell at constant temperature, a rise or fall in temperature causes a fall in intracellular cAMP levels within seconds, followed by loss of adherence to the culture dish and retraction of cell processes. The mechanism of this fall in cAMP level is at least in part excretion into the medium. The decrease in adhesiveness and retraction of processes can be blocked by analogues of cAMP or agents that elevate intracellular cAMP. The properties of this mutant imply that cAMP is a direct regulator of cell shape and adhesiveness.
M C Willingham, and
R A Carchman, and
I H Pastan
July 1973,
Experimental cell research,
M C Willingham, and
R A Carchman, and
I H Pastan
October 1980,
In vitro,
M C Willingham, and
R A Carchman, and
I H Pastan
July 1981,
Proceedings of the National Academy of Sciences of the United States of America,
M C Willingham, and
R A Carchman, and
I H Pastan
July 1976,
FEBS letters,
M C Willingham, and
R A Carchman, and
I H Pastan
February 1976,
Nature,
M C Willingham, and
R A Carchman, and
I H Pastan
May 1995,
Biochemical Society transactions,
M C Willingham, and
R A Carchman, and
I H Pastan
September 1989,
Biochimica et biophysica acta,
M C Willingham, and
R A Carchman, and
I H Pastan
January 1981,
Advances in cyclic nucleotide research,
M C Willingham, and
R A Carchman, and
I H Pastan
September 1975,
Nature,
M C Willingham, and
R A Carchman, and
I H Pastan
August 1989,
Biochemical Society transactions,
