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A simple and inexpensive assay method of superoxide dismutase (SOD) activity of various biological materials has been developed. SOD activity can easily be measured by reading the medium's intense absorbance directly, and many samples can be treated in a short time using a reaction stopper which acts for a long time. Reproducibility of the assay method was excellent for the sample from an individual estimated at two different times (a week to a month) and the coefficient of variance was 5.1%. The mean and standard deviation of SOD activities of 45 blood samples from normal Japanese males 32.9 +/- 10.5 microgram per ml of blood; estimated using bovine SOD standard. The SOD activity of the fluid from lavaged lungs was 15.7 +/- 1.8 microgram/ml, and that of lung tissue homogenates from rats was 2.85 +/- 0.66 microgram/mg of protein.
M Minami, and
H Yoshikawa
March 1988,
Clinical chemistry,
M Minami, and
H Yoshikawa
June 1989,
Clinical chemistry,
M Minami, and
H Yoshikawa
January 1988,
Free radical biology & medicine,
M Minami, and
H Yoshikawa
December 1988,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
M Minami, and
H Yoshikawa
September 1981,
Analytical biochemistry,
M Minami, and
H Yoshikawa
March 1982,
Journal of biochemistry,
M Minami, and
H Yoshikawa
January 1984,
Methods in enzymology,
M Minami, and
H Yoshikawa
January 2002,
Methods in enzymology,
M Minami, and
H Yoshikawa
December 1982,
Analytical biochemistry,
M Minami, and
H Yoshikawa
January 1985,
Zeitschrift fur medizinische Laboratoriumsdiagnostik,
