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Friend leukemia cells (GM86, clone 745) were induced to differentiate with dimethyl sulfoxide or butyric acid. The kinetics of induction were measured by cell growth, cell volume distributions, and [3H]thymidine incorporation. From the volume distributions, it was found that the rate of induction was both agent sensitive and concentration dependent. The changes in volume distributions occurred approximately 4 hr earlier with dimethyl sulfoxide induction relative to butyric acid induction. However, the changes with the butyric acid induction were more dependent on concentration. A decrease in labeling indices during the 12- to 20-hr time period was correlated to a decrease in mean cell volume and an increase in the proportion of G1 cells. After the 20-hr time period of induction, an increase in labeling indices and in the percentage of large cells was observed. The data suggest that a transient block of cells in G1 occurred between 12 to 20 hr, and that the early differentiation involved a volume decrease which was related to a redistribution of cell cycle stages. The study was also shown that the changes of cell volume are a rapid monitor to determine the early events of the differentiation process.
R M Zucker, and
N C Wu, and
A Mitrani, and
M Silverman
September 1979,
Diabetes,
R M Zucker, and
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M Silverman
December 1979,
Biochimica et biophysica acta,
R M Zucker, and
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August 1982,
Biochemical and biophysical research communications,
R M Zucker, and
N C Wu, and
A Mitrani, and
M Silverman
August 1980,
Nucleic acids research,
R M Zucker, and
N C Wu, and
A Mitrani, and
M Silverman
October 1993,
Biochimica et biophysica acta,
R M Zucker, and
N C Wu, and
A Mitrani, and
M Silverman
January 1980,
Molecular & general genetics : MGG,
R M Zucker, and
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M Silverman
October 1990,
Leukemia,
R M Zucker, and
N C Wu, and
A Mitrani, and
M Silverman
December 1975,
Cell differentiation,
R M Zucker, and
N C Wu, and
A Mitrani, and
M Silverman
August 1980,
Nucleic acids research,
R M Zucker, and
N C Wu, and
A Mitrani, and
M Silverman
March 1979,
Cancer research,
