BIOMAT Database Logo BIOMAT Database Logo

Dihydrofolate reductase from amethopterin-resistant Lactobacillus casei. 1972

L E Gundersen, and R B Dunlap, and N G Harding, and J H Freisheim, and F Otting, and F M Huennekens

UI MeSH Term Description Entries
D007778 Lactobacillus A genus of gram-positive, microaerophilic, rod-shaped bacteria occurring widely in nature. Its species are also part of the many normal flora of the mouth, intestinal tract, and vagina of many mammals, including humans. Lactobacillus species are homofermentative and ferment a broad spectrum of carbohydrates often host-adapted but do not ferment PENTOSES. Most members were previously assigned to the Lactobacillus delbrueckii group. Pathogenicity from this genus is rare.
D007780 Lacticaseibacillus casei A rod-shaped bacterium isolated from milk and cheese, dairy products and dairy environments, sour dough, cow dung, silage, and human mouth, human intestinal contents and stools, and the human vagina. L. casei is CATALASE positive. Lactobacillus casei
D008727 Methotrexate An antineoplastic antimetabolite with immunosuppressant properties. It is an inhibitor of TETRAHYDROFOLATE DEHYDROGENASE and prevents the formation of tetrahydrofolate, necessary for synthesis of thymidylate, an essential component of DNA. Amethopterin,Methotrexate Hydrate,Methotrexate Sodium,Methotrexate, (D)-Isomer,Methotrexate, (DL)-Isomer,Methotrexate, Dicesium Salt,Methotrexate, Disodium Salt,Methotrexate, Sodium Salt,Mexate,Dicesium Salt Methotrexate,Hydrate, Methotrexate,Sodium, Methotrexate
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D009249 NADP Nicotinamide adenine dinucleotide phosphate. A coenzyme composed of ribosylnicotinamide 5'-phosphate (NMN) coupled by pyrophosphate linkage to the 5'-phosphate adenosine 2',5'-bisphosphate. It serves as an electron carrier in a number of reactions, being alternately oxidized (NADP+) and reduced (NADPH). (Dorland, 27th ed) Coenzyme II,Nicotinamide-Adenine Dinucleotide Phosphate,Triphosphopyridine Nucleotide,NADPH,Dinucleotide Phosphate, Nicotinamide-Adenine,Nicotinamide Adenine Dinucleotide Phosphate,Nucleotide, Triphosphopyridine,Phosphate, Nicotinamide-Adenine Dinucleotide
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D002845 Chromatography Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts. Chromatographies
D002850 Chromatography, Gel Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. Chromatography, Exclusion,Chromatography, Gel Permeation,Chromatography, Molecular Sieve,Gel Filtration,Gel Filtration Chromatography,Chromatography, Size Exclusion,Exclusion Chromatography,Gel Chromatography,Gel Permeation Chromatography,Molecular Sieve Chromatography,Chromatography, Gel Filtration,Exclusion Chromatography, Size,Filtration Chromatography, Gel,Filtration, Gel,Sieve Chromatography, Molecular,Size Exclusion Chromatography
D002852 Chromatography, Ion Exchange Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins. Chromatography, Ion-Exchange,Ion-Exchange Chromatography,Chromatographies, Ion Exchange,Chromatographies, Ion-Exchange,Ion Exchange Chromatographies,Ion Exchange Chromatography,Ion-Exchange Chromatographies
D004352 Drug Resistance, Microbial The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS). Antibiotic Resistance,Antibiotic Resistance, Microbial,Antimicrobial Resistance, Drug,Antimicrobial Drug Resistance,Antimicrobial Drug Resistances,Antimicrobial Resistances, Drug,Drug Antimicrobial Resistance,Drug Antimicrobial Resistances,Drug Resistances, Microbial,Resistance, Antibiotic,Resistance, Drug Antimicrobial,Resistances, Drug Antimicrobial

Related Publications

L E Gundersen, and R B Dunlap, and N G Harding, and J H Freisheim, and F Otting, and F M Huennekens
Amino acid sequence of dihydrofolate reductase from an amethopterin-resistant strain of Lactobacillus casei.
August 1977, FEBS letters,
L E Gundersen, and R B Dunlap, and N G Harding, and J H Freisheim, and F Otting, and F M Huennekens
TPNH-dependent binding of amethopterin by dihydrofolate reductase from Lactobacillus casei.
September 1972, Archives of biochemistry and biophysics,
L E Gundersen, and R B Dunlap, and N G Harding, and J H Freisheim, and F Otting, and F M Huennekens
Interaction of dihydrofolate reductase from amethopterin-resistant Lactobacillus casei with Cibacron Blue, Blue Dextran, and Affi-Gel Blue.
July 1979, The Journal of biological chemistry,
L E Gundersen, and R B Dunlap, and N G Harding, and J H Freisheim, and F Otting, and F M Huennekens
Thymidylate synthetase from amethopterin-resistant Lactobacillus casei.
January 1971, Biochemistry,
L E Gundersen, and R B Dunlap, and N G Harding, and J H Freisheim, and F Otting, and F M Huennekens
Dihydrofolate reductase from amethopterin-resistant Lactobacillus casei. Sequences of the cyanogen bromide peptides and complete sequences of the enzyme.
September 1978, The Journal of biological chemistry,
L E Gundersen, and R B Dunlap, and N G Harding, and J H Freisheim, and F Otting, and F M Huennekens
Reduction of oxidised folates by dihydrofolate reductase from methotrexate-resistant Lactobacillus casei.
June 1977, Biochimica et biophysica acta,
L E Gundersen, and R B Dunlap, and N G Harding, and J H Freisheim, and F Otting, and F M Huennekens
DNA binding by dihydrofolate reductase from Lactobacillus casei.
December 1981, The Journal of biological chemistry,
L E Gundersen, and R B Dunlap, and N G Harding, and J H Freisheim, and F Otting, and F M Huennekens
Denaturation of thymidylate synthetase from amethopterin-resistant Lactobacillus casei.
January 1979, Cancer biochemistry biophysics,
L E Gundersen, and R B Dunlap, and N G Harding, and J H Freisheim, and F Otting, and F M Huennekens
Dihydrofolate reductase from Lactobacillus casei. Stereochemistry of NADPH binding.
May 1979, The Journal of biological chemistry,
L E Gundersen, and R B Dunlap, and N G Harding, and J H Freisheim, and F Otting, and F M Huennekens
Dihydrofolate reductase from amethopterin-resistant Lactobacillus casei. Effects of pH, salts, temperature, and source of NADPH on enzyme activity and substrate specificity studies.
June 1977, Archives of biochemistry and biophysics,
Copied contents to your clipboard!