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Ultrastructure of molluscum contagiosum virus as revealed by freeze-etching technique. 1974

T Hasegawa, and M Takeuchi, and S Imamura

UI MeSH Term Description Entries
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D008977 Molluscum contagiosum virus A species of MOLLUSCIPOXVIRUS causing skin lesions in humans. It is transmitted by direct contact or from non-living reservoirs (fomites), such as books or clothing. Molluscum contagiosum viruses
D005613 Freeze Etching A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces. Etching, Freeze

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