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Membrane vesicles from Mycobacterium phlei contain carrier proteins for proline, glutamine, and glutamic acid. The transport of proline is Na+ dependent and required substrate oxidation. A proline carrier protein was solubilized from the membrane vesicles by treatment with cholate and Triton X-100. Electron microscopic observation of the detergent-treated membrane vesicles showed that they are closed structures. The detergent-extracted proteins were purified by means of sucrose density gradient centrifugation, followed by gel filtration and isoelectric focusing. A single protein with a molecular weight of 20,000 +/- 1000 was found on polyacrylamide gel electrophoresis. Reconstitution of proline transport was demonstrated when the purified protein was incubated with the detergent-extracted membrane vesicles. This reconstituted transport system was specific for proline and required substrate oxidation and Na+. The purified protein was also incorporated into liposomes, and proline uptake was demonstrated when energy was supplied as a membrane potential introduced by K+ diffusion via valinomycin. The uptake of proline was Na+ dependent and was inhibited by uncoupler or by sulfhydryl reagents.
S H Lee, and
N S Cohen, and
A J Jacobs, and
A F Brodie
November 1978,
Biochemical and biophysical research communications,
S H Lee, and
N S Cohen, and
A J Jacobs, and
A F Brodie
February 1970,
Biochemical and biophysical research communications,
S H Lee, and
N S Cohen, and
A J Jacobs, and
A F Brodie
January 1970,
Biochemical and biophysical research communications,
S H Lee, and
N S Cohen, and
A J Jacobs, and
A F Brodie
February 1998,
FEMS microbiology letters,
S H Lee, and
N S Cohen, and
A J Jacobs, and
A F Brodie
January 2013,
Bioinformation,
S H Lee, and
N S Cohen, and
A J Jacobs, and
A F Brodie
May 1988,
The Journal of biological chemistry,
S H Lee, and
N S Cohen, and
A J Jacobs, and
A F Brodie
January 1975,
Osaka city medical journal,
S H Lee, and
N S Cohen, and
A J Jacobs, and
A F Brodie
August 1979,
The Journal of biological chemistry,
S H Lee, and
N S Cohen, and
A J Jacobs, and
A F Brodie
January 1971,
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Zweite naturwissenschaftliche Abt.: Allgemeine, landwirtschaftliche und technische Mikrobiologie,
S H Lee, and
N S Cohen, and
A J Jacobs, and
A F Brodie
June 1977,
Biochimica et biophysica acta,
