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In peripheral human blood lymphocytes the uptake and metabolism of adenine, guanine, and hypoxanthine was investigated. This was achieved by incubation of purified lymphocytes with 14C-purine bases, separation of cells from the incubation medium by a rapid filtration technique, and subsequent separation of the acid soluble material by thin-layer chromatography. No perferential uptake for one of the purine bases was observed. In all cases only traces of 14C-purine bases not added originally and labeled nucleosides could be demonstrated. Approximately 2/3 of adenine and 1/2 of guanine or hypoxanthine were converted to nucleotides. Separation of formed nucleotides showed that adenine and guanine were metabolized mainly to their corresponding nucleotides; hypoxanthine was converted to a considerable amount to adenine nucleotides and only to a small proportion into its own nucleotides. These results demonstrate the predomonance of adenine nucleotide formation in normal human lymphocytes.
M M Müller, and
G Pischek, and
O Scheiner, and
H Stemberger, and
G Wiedermann
January 1980,
Journal of immunological methods,
M M Müller, and
G Pischek, and
O Scheiner, and
H Stemberger, and
G Wiedermann
July 1984,
Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire,
M M Müller, and
G Pischek, and
O Scheiner, and
H Stemberger, and
G Wiedermann
January 1984,
International journal of tissue reactions,
M M Müller, and
G Pischek, and
O Scheiner, and
H Stemberger, and
G Wiedermann
April 1990,
Bollettino della Societa italiana di biologia sperimentale,
M M Müller, and
G Pischek, and
O Scheiner, and
H Stemberger, and
G Wiedermann
October 1987,
Archives of biochemistry and biophysics,
M M Müller, and
G Pischek, and
O Scheiner, and
H Stemberger, and
G Wiedermann
August 1991,
Biochemical Society transactions,
M M Müller, and
G Pischek, and
O Scheiner, and
H Stemberger, and
G Wiedermann
October 1983,
The Journal of biological chemistry,
M M Müller, and
G Pischek, and
O Scheiner, and
H Stemberger, and
G Wiedermann
September 1985,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
M M Müller, and
G Pischek, and
O Scheiner, and
H Stemberger, and
G Wiedermann
January 2000,
Advances in experimental medicine and biology,
M M Müller, and
G Pischek, and
O Scheiner, and
H Stemberger, and
G Wiedermann
January 1983,
The International journal of biochemistry,
