Quantitation of proteins by electroimmunoassay using a digitizer connected with a programmable calculator. 1979

I Andersen

A system is described which considerably facilitates the reading and the subsequent conversion of measured values to protein concentrations, when proteins are quantitated by the electroimmunoassay a.m. Laurell (1972). The rocket heights of calibration samples and unknown samples are read by a cursor on a magnetic table (Digitizer, Hewlett Packard) and the values are automatically transferred to a programmable calculator (HP 9830 A, Hewlett Packard). It is programmed to calculate the protein concentration of samples by interpolation on a calibration curve fitted to the best polynomium of second degree by the method of least squares. The results and sequence numbers are automatically printed out from a printer (HP 9866 A, Hewlett, Packard), Reading and calculation of the results from one plate with 5 calibration samples (in duplicate) and 20 unknown samples are completed in less than 2 min. This is 10--15 times faster compared with a manual procedure where a hand-drawn calibration curve is used for interpolation.

UI MeSH Term Description Entries
D007122 Immunoelectrophoresis A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
D011506 Proteins Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein. Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D003201 Computers Programmable electronic devices designed to accept data, perform prescribed mathematical and logical operations at high speed, and display the results of these operations. Calculators, Programmable,Computer Hardware,Computers, Digital,Hardware, Computer,Calculator, Programmable,Computer,Computer, Digital,Digital Computer,Digital Computers,Programmable Calculator,Programmable Calculators

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