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Following ultracentrifugation in sucrose and hypotonic exposure ('osmotic shock'), myelin fractions were prepared from homogenates of human centrum ovale obtained post-mortem. Non-specific esterase (NsE), succinic dehydrogenase and acid phosphatase activities were determined. One isolation procedure utilized aqueous sucrose solutions only. Other preparative technics involved addition of phosphate buffer (pH 6.6 or 8.0) or varying concentrations of calcium or magnesium ions to preparative media. The NsE activity of myelin increased greatly when electrolytes were included in these preparative solutions. Morphologic homogeneity and protein and lipid contents of myelin were similar whatever the isolation procedure.
E J Mitzen, and
K D Barron, and
A H Koeppen, and
H W Harris
January 1970,
Acta neurologica Scandinavica,
E J Mitzen, and
K D Barron, and
A H Koeppen, and
H W Harris
April 1975,
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E J Mitzen, and
K D Barron, and
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H W Harris
October 1978,
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E J Mitzen, and
K D Barron, and
A H Koeppen, and
H W Harris
May 1996,
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E J Mitzen, and
K D Barron, and
A H Koeppen, and
H W Harris
January 1970,
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E J Mitzen, and
K D Barron, and
A H Koeppen, and
H W Harris
December 1972,
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E J Mitzen, and
K D Barron, and
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H W Harris
March 1983,
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E J Mitzen, and
K D Barron, and
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H W Harris
February 1969,
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E J Mitzen, and
K D Barron, and
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H W Harris
January 1980,
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E J Mitzen, and
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November 2016,
Neuropharmacology,
