-transparent.png){kind=logo}
Bovine prothrombin contains three asparagine-linked sugar chains in 1 molecule. The sugar chains were quantitatively released from the polypeptide backbone by hydrazinolysis. All of the oligosaccharides thus obtained contain N-acetylneuraminic acid. Sialidase treatment of these acidic oligosaccharides released three isomeric oligosaccharides, N-1, N-2 and N-3. N-3 was a typical complex type asparagine-linked sugar chain widely found in other glycoprotein, while N-1 and N-2 were unique, because they contain Gal beta 1 leads to 3GlcNAc grouping in the outer chain moiety. By comparing the data of methylation analysis of the acidic oligosaccharides before and after sialidase treatment, the structures of the sugar chains of bovine prothrombin were confirmed as a mixture of NeuAc alpha 2 leads to 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 6(NeuAc alpha 2 leads to 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 3)Man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc leads to Asn, NeuAc alpha 2 leads to 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 6[NeuAc alpha 2 leads to 3Gal beta 1 leads to 3(NeuAc alpha 2 leads to 6)GlcNAc beta 1 leads to 2Man alpha 1 leads to 3]Man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc leads to Asn, NeuAc alpha 2 leads to 3Gal beta 1 leads to 3(NeuAc alpha 2 leads to 6)GlcNAc beta 1 leads to 2Man alpha 1 leads to 6[NeuAc alpha 2 leads to 3Gal beta 1 leads to 3(NeuAc alpha 2 leads to 6)GlcNAc beta 1 leads to 2Man alpha 1 leads to 3]Man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc leads to Asn and their partially desialized forms.
T Mizuochi, and
K Yamashita, and
K Fujikawa, and
W Kisiel, and
A Kobata
September 1986,
Biochemistry,
T Mizuochi, and
K Yamashita, and
K Fujikawa, and
W Kisiel, and
A Kobata
February 1991,
Infection and immunity,
T Mizuochi, and
K Yamashita, and
K Fujikawa, and
W Kisiel, and
A Kobata
October 1989,
The Journal of biological chemistry,
T Mizuochi, and
K Yamashita, and
K Fujikawa, and
W Kisiel, and
A Kobata
March 1993,
Molecular immunology,
T Mizuochi, and
K Yamashita, and
K Fujikawa, and
W Kisiel, and
A Kobata
January 1989,
Glycoconjugate journal,
T Mizuochi, and
K Yamashita, and
K Fujikawa, and
W Kisiel, and
A Kobata
January 1990,
Carbohydrate research,
T Mizuochi, and
K Yamashita, and
K Fujikawa, and
W Kisiel, and
A Kobata
September 1985,
Biochemistry,
T Mizuochi, and
K Yamashita, and
K Fujikawa, and
W Kisiel, and
A Kobata
December 1993,
Journal of biochemistry,
T Mizuochi, and
K Yamashita, and
K Fujikawa, and
W Kisiel, and
A Kobata
February 1986,
The Journal of biological chemistry,
T Mizuochi, and
K Yamashita, and
K Fujikawa, and
W Kisiel, and
A Kobata
August 1986,
Biochimica et biophysica acta,
