-transparent.png){kind=logo}
To evaluate the ultrastructural distribution of transferrin on the surface of L1210 ascites tumor cells, we used ferrocyanide to stain ferric iron (Prussian blue reaction) in transferrin, as well as in ferritin conjugated to antibody that was immunologically attached to the transferrin. Small deposits averaging 5 nm in diameter identified transferrin iron, whereas large cuboidal deposits averaging 50 nm in diameter stained ferritin conjugated-antibody that was bound to both transferrin and apotransferrin on the cell surface. The ability of transferrin to deliver iron to ascites tumor cells was confirmed by kinetic studies of transferrin labeled with 59Fe and 125I. These preliminary results are consistent with release of transferrin iron at the cell surface and demonstrate additional uses for ferrocyanide in ultrastructural cytochemical techniques.
R T Parmley, and
F Ostroy, and
R A Gams, and
L DeLucas
January 1976,
Blood,
R T Parmley, and
F Ostroy, and
R A Gams, and
L DeLucas
July 1979,
The Histochemical journal,
R T Parmley, and
F Ostroy, and
R A Gams, and
L DeLucas
January 1966,
Wakayama medical reports,
R T Parmley, and
F Ostroy, and
R A Gams, and
L DeLucas
December 1972,
Virology,
R T Parmley, and
F Ostroy, and
R A Gams, and
L DeLucas
January 1971,
Medical microbiology and immunology,
R T Parmley, and
F Ostroy, and
R A Gams, and
L DeLucas
September 1960,
Nature,
R T Parmley, and
F Ostroy, and
R A Gams, and
L DeLucas
May 1999,
International journal of pharmaceutics,
R T Parmley, and
F Ostroy, and
R A Gams, and
L DeLucas
June 1967,
The Journal of laboratory and clinical medicine,
R T Parmley, and
F Ostroy, and
R A Gams, and
L DeLucas
June 1972,
Nihon rinsho. Japanese journal of clinical medicine,
R T Parmley, and
F Ostroy, and
R A Gams, and
L DeLucas
June 1967,
The Journal of parasitology,
