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The effect of donor age on the rate of cell entry into the proliferating pool and subsequent cell cycle duration for peripheral lymphocytes stimulated by phytohemagglutinin (PHA) were examined by using the bromodeoxyuridine incorporation-differential staining technique. Distribution curves for the appearance of metaphase cells in successive generations as a function of culture time were obtained and analyzed both graphically and by a computer simulation model. Peripheral lymphocytes from aged individuals (approximately 75 yr) were stimulated by PHA at approximately one-half of the rate of peripheral lymphocytes from young individuals (approximately 21 yr). Subsequent cell-cycle durations were estimated to range from 10.0 to 25.0 h for aged individual lymphocyte cultures and 10.6-15.6 h for young individual lymphocyte cultures. The possible significance of these findings to aging in general is discussed.
R R Tice, and
E L Schneider, and
D Kram, and
P Thorne
May 1977,
The Journal of experimental medicine,
R R Tice, and
E L Schneider, and
D Kram, and
P Thorne
January 1987,
Chemico-biological interactions,
R R Tice, and
E L Schneider, and
D Kram, and
P Thorne
September 1976,
Clinical immunology and immunopathology,
R R Tice, and
E L Schneider, and
D Kram, and
P Thorne
June 1991,
Zeitschrift fur Gastroenterologie,
R R Tice, and
E L Schneider, and
D Kram, and
P Thorne
January 1967,
International archives of allergy and applied immunology,
R R Tice, and
E L Schneider, and
D Kram, and
P Thorne
January 1966,
Federation proceedings,
R R Tice, and
E L Schneider, and
D Kram, and
P Thorne
July 1986,
The Journal of clinical investigation,
R R Tice, and
E L Schneider, and
D Kram, and
P Thorne
February 1969,
Journal of the Reticuloendothelial Society,
R R Tice, and
E L Schneider, and
D Kram, and
P Thorne
April 1975,
Cellular immunology,
R R Tice, and
E L Schneider, and
D Kram, and
P Thorne
December 1992,
Annals of the New York Academy of Sciences,
