BIOMAT Database Logo BIOMAT Database Logo

A factor from a transformed cell line that affects cell migration. 1973

R R Bürk

When a monolayer culture of normal Balb/c3T3 cells is wounded by scraping away part of the cell sheet, the cells do not migrate into the cleared area unless there is serum in the culture medium. By contrast, SV40-transformed Balb/c3T3 cells do migrate into the wound area without serum. A quantitative assay for the migration of Balb/c3T3 cells into wounds is described. This assay is used in the partial purification of a migration factor released into serum-free medium by SV28 cells. SV28 is a line of BHK21/13 hamster cells transformed by SV40 chosen for its malignancy. The most purified fractions have about 1500 times the specific activity of whole calf serum. These fractions have an activity that promotes overgrowth of Balb/c3T3 cells to high density and an activity that prolongs cell survival without serum. The SV28 migration factor is not extractable from the medium of untransformed BHK21/13 cells or from serum. This migration factor might contribute to the malignancy of SV28 cells.

UI MeSH Term Description Entries
D001769 Blood The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002465 Cell Movement The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell. Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D002471 Cell Transformation, Neoplastic Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill. Neoplastic Transformation, Cell,Neoplastic Cell Transformation,Transformation, Neoplastic Cell,Tumorigenic Transformation,Cell Neoplastic Transformation,Cell Neoplastic Transformations,Cell Transformations, Neoplastic,Neoplastic Cell Transformations,Neoplastic Transformations, Cell,Transformation, Cell Neoplastic,Transformation, Tumorigenic,Transformations, Cell Neoplastic,Transformations, Neoplastic Cell,Transformations, Tumorigenic,Tumorigenic Transformations
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002850 Chromatography, Gel Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. Chromatography, Exclusion,Chromatography, Gel Permeation,Chromatography, Molecular Sieve,Gel Filtration,Gel Filtration Chromatography,Chromatography, Size Exclusion,Exclusion Chromatography,Gel Chromatography,Gel Permeation Chromatography,Molecular Sieve Chromatography,Chromatography, Gel Filtration,Exclusion Chromatography, Size,Filtration Chromatography, Gel,Filtration, Gel,Sieve Chromatography, Molecular,Size Exclusion Chromatography
D003260 Contact Inhibition Arrest of cell locomotion or cell division when two cells come into contact. Inhibition, Contact,Contact Inhibitions,Inhibitions, Contact
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D006224 Cricetinae A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS. Cricetus,Hamsters,Hamster

Related Publications

R R Bürk
Isolation method affects transformed cell line karyotype.
March 1978, Nature,
R R Bürk
Acetylcholine receptor-specific suppressive T-cell factor from a retrovirally transformed T-cell line.
December 1984, Proceedings of the National Academy of Sciences of the United States of America,
R R Bürk
Regulation of ornithine decarboxylase in a transformed cell line that overexpresses translation initiation factor eIF-4E.
July 1996, Cancer research,
R R Bürk
A transformed human epithelial cell line that retains tight junctions post crisis.
January 1992, In vitro cellular & developmental biology : journal of the Tissue Culture Association,
R R Bürk
Prenatal Environment That Affects Neuronal Migration.
January 2019, Frontiers in cell and developmental biology,
R R Bürk
Identification of hPin1 inhibitors that induce apoptosis in a mammalian Ras transformed cell line.
January 2005, Chemical communications (Cambridge, England),
R R Bürk
Ectopic peptides released by a human melanoma cell line that modulate the transformed phenotype.
January 1985, Ciba Foundation symposium,
R R Bürk
A transformed human tracheal gland cell line, MM-39, that retains serous secretory functions.
October 1996, American journal of respiratory cell and molecular biology,
R R Bürk
Ectopic peptides released by a human melanoma cell line that modulate the transformed phenotype.
August 1985, Proceedings of the National Academy of Sciences of the United States of America,
R R Bürk
Platelet-derived growth factor-modulated proteins: constitutive synthesis by a transformed cell line.
July 1981, Proceedings of the National Academy of Sciences of the United States of America,
Copied contents to your clipboard!