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The structure of the Fab' fragment of a human myeloma immunoglobulin was determined by x-ray crystallographic analysis at 2.8-A resolution. The Fourier map of the electron density was correlated with the aminoacid sequence to obtain a three-dimensional model. Four globular subunits, which correspond to the homology regions of the light and heavy chains, are arranged in a tetrahedral configuration. These subunits closely resemble each other, sharing a basic pattern of polypeptide chain folding. In each subunit, long sequences of tightly packed, hydrogen bonded polypeptide chain run parallel to the major axis of the subunit. No helical conformation can be seen. Different patterns of interchain disulfide linkage and unusual intrachain disulfide bonds that have been observed in other immunoglobulins can be explained with this model. The regions of hypervariable sequences in the light and heavy chains occur at one end of the molecule, in close spatial proximity.
R J Poljak, and
L M Amzel, and
H P Avey, and
B L Chen, and
R P Phizackerley, and
F Saul
November 1992,
Proteins,
R J Poljak, and
L M Amzel, and
H P Avey, and
B L Chen, and
R P Phizackerley, and
F Saul
January 2004,
Bioorganicheskaia khimiia,
R J Poljak, and
L M Amzel, and
H P Avey, and
B L Chen, and
R P Phizackerley, and
F Saul
June 1971,
The Journal of biological chemistry,
R J Poljak, and
L M Amzel, and
H P Avey, and
B L Chen, and
R P Phizackerley, and
F Saul
January 1987,
The Journal of biological chemistry,
R J Poljak, and
L M Amzel, and
H P Avey, and
B L Chen, and
R P Phizackerley, and
F Saul
September 1992,
Protein science : a publication of the Protein Society,
R J Poljak, and
L M Amzel, and
H P Avey, and
B L Chen, and
R P Phizackerley, and
F Saul
September 1994,
Acta crystallographica. Section D, Biological crystallography,
R J Poljak, and
L M Amzel, and
H P Avey, and
B L Chen, and
R P Phizackerley, and
F Saul
August 1979,
Proceedings of the National Academy of Sciences of the United States of America,
R J Poljak, and
L M Amzel, and
H P Avey, and
B L Chen, and
R P Phizackerley, and
F Saul
December 2000,
Journal of immunology (Baltimore, Md. : 1950),
R J Poljak, and
L M Amzel, and
H P Avey, and
B L Chen, and
R P Phizackerley, and
F Saul
May 1995,
Acta crystallographica. Section D, Biological crystallography,
R J Poljak, and
L M Amzel, and
H P Avey, and
B L Chen, and
R P Phizackerley, and
F Saul
July 1998,
Journal of molecular biology,
