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A procedure for screening several thousand clones for alterations in a specific gene product was apapplied to mutagenized cultures of Dictyostelium discoideum. Six strains were recovered which had less N-acetylglucosaminidase (EC 3.2.1.30) than the wild type. In addition, we isolated four strains in which the enzyme was temperature-sensitive. The enzyme from one of these strains had an altered substrate affinity.N-Acetylglucosaminidase is present at a low level in cells grown on bacteria and increases up to 10-fold during the aggregation stage of development. The enzyme appears to be essential to maintain migrating pseudoplasmodia of normal size, as shown by the fact that all of the mutant strains which accumulate very little N-acetyl-glucosaminidase formed only small migrating pseudoplasmodia containing less than 10% the number of cells found in the wild-type pseudoplasmodia. The small pseudoplasmodia migrated at less than a third the rate of wild-type pseudoplasmodia and ultimately formed diminutive fruiting bodies. N-Acetylglucosaminidase thus appears to be a developmental enzyme that functions during the migration stage.
R L Dimond, and
M Brenner, and
W F Loomis
September 1974,
The Journal of biological chemistry,
R L Dimond, and
M Brenner, and
W F Loomis
February 1978,
Genetics,
R L Dimond, and
M Brenner, and
W F Loomis
July 1980,
Developmental biology,
R L Dimond, and
M Brenner, and
W F Loomis
June 1978,
Developmental biology,
R L Dimond, and
M Brenner, and
W F Loomis
January 1974,
Biochimie,
R L Dimond, and
M Brenner, and
W F Loomis
March 1984,
Molecular and cellular biology,
R L Dimond, and
M Brenner, and
W F Loomis
December 1988,
Genetics,
R L Dimond, and
M Brenner, and
W F Loomis
March 1969,
Journal of bacteriology,
R L Dimond, and
M Brenner, and
W F Loomis
July 1984,
Archives of biochemistry and biophysics,
R L Dimond, and
M Brenner, and
W F Loomis
May 1976,
Developmental biology,
