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Sedimentation equilibrium was achieved with turnip yellow mosaic virus at low speeds (600 rpm) in a magnetic ultracentrifuge. The experiments were carried out in the newly installed constant-speed rotor, equipped with automatic control and an electromagnetic drive. A particle mass of 5.55 x 10(6) daltons was calculated for the virus at vanishing concentrations, in essential agreement with the earlier results using the more tedious procedure with a freely coasting rotor. In order to interpret the observed departure from ideal behavior (nonassociating conditions), preferential interaction experiments were carried out by magnetic densimetry. These showed that a strong Donnan effect exists at pH 7, which contributed > 10(3) times more to the nonideality than did the excluded volume effect. A net negative charge of about 3.5 x 10(3) per particle was estimated at this pH.
J H McGee, and
D W Kupke, and
W Godschalk, and
J W Beams
January 1966,
Advances in virus research,
J H McGee, and
D W Kupke, and
W Godschalk, and
J W Beams
March 1957,
Nature,
J H McGee, and
D W Kupke, and
W Godschalk, and
J W Beams
May 1952,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
J H McGee, and
D W Kupke, and
W Godschalk, and
J W Beams
August 1966,
Journal of molecular biology,
J H McGee, and
D W Kupke, and
W Godschalk, and
J W Beams
September 1996,
Nature structural biology,
J H McGee, and
D W Kupke, and
W Godschalk, and
J W Beams
January 1981,
Intervirology,
J H McGee, and
D W Kupke, and
W Godschalk, and
J W Beams
April 1960,
Nature,
J H McGee, and
D W Kupke, and
W Godschalk, and
J W Beams
February 1949,
Parasitology,
J H McGee, and
D W Kupke, and
W Godschalk, and
J W Beams
May 2009,
Langmuir : the ACS journal of surfaces and colloids,
J H McGee, and
D W Kupke, and
W Godschalk, and
J W Beams
February 1961,
Journal of molecular biology,
