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Human/mouse microcell hybrids were used to establish the assignment of the gene governing resistance to the cardiac glycoside ouabain (Oua-1) to Mus musculus chromosome 3. Microcells were prepared from primary mouse embryo fibroblasts and fused with HeLa S3 cells, and microcell hybrids were isolated and maintained in medium containing 10(-6) M ouabain. Resistance to ouabain was not expressed concordantly with any of 26 murine isozyme markers. Karyotypic analysis of five primary clones showed that one to five murine chromosomes had been transferred from donor to recipient in these experiments. Only mouse chromosome 3 was common to all ouabain-resistant primary clones. Both ouabain-resistant and -sensitive subclones were isolated from hybrids grown in the absence of selective pressure, and karyotyping showed that loss of resistance to ouabain was concordant with the loss of murine chromosome 3.
C A Kozak, and
R E Fournier, and
L A Leinwand, and
F H Ruddle
March 1978,
Somatic cell genetics,
C A Kozak, and
R E Fournier, and
L A Leinwand, and
F H Ruddle
January 1983,
Somatic cell genetics,
C A Kozak, and
R E Fournier, and
L A Leinwand, and
F H Ruddle
January 1978,
The Journal of heredity,
C A Kozak, and
R E Fournier, and
L A Leinwand, and
F H Ruddle
January 1978,
Cytogenetics and cell genetics,
C A Kozak, and
R E Fournier, and
L A Leinwand, and
F H Ruddle
November 1985,
Somatic cell and molecular genetics,
C A Kozak, and
R E Fournier, and
L A Leinwand, and
F H Ruddle
January 1978,
Cytogenetics and cell genetics,
C A Kozak, and
R E Fournier, and
L A Leinwand, and
F H Ruddle
May 1992,
Genomics,
C A Kozak, and
R E Fournier, and
L A Leinwand, and
F H Ruddle
June 1978,
Biochemical genetics,
C A Kozak, and
R E Fournier, and
L A Leinwand, and
F H Ruddle
January 1977,
Cytogenetics and cell genetics,
C A Kozak, and
R E Fournier, and
L A Leinwand, and
F H Ruddle
January 1985,
Nature,
