Biomaterial Database

Mass spectrometric determination of hydroxylamine photooxidation by illuminated chloroplasts. 1979

R Radmer

A mass spectrometer with a special inlet was used to directly monitor the products evolved when hydroxylamine-treated chloroplasts were exposed to short saturating light flashes. We found that: 1. Molecular dinitrogen was the sole product of hydroxylamine photooxidation, and was formed in an amount equal to twice the O2 evolved during H2O photooxidation. 2. This reaction was driven by Photosystem II, and did not involve Photo-system I-generated superoxide or peroxide. 3. In the presence of 3-(3,4-dichlorophenyl)-1,1-dimethyl urea, N2 was evolved only on the first flash. These results suggested that N2 was formed by the combination of two single-electron oxidation products of hydroxylamine.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008027	Light	That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.	Light, Visible,Photoradiation,Radiation, Visible,Visible Radiation,Photoradiations,Radiations, Visible,Visible Light,Visible Radiations
D010084	Oxidation-Reduction	A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).	Redox,Oxidation Reduction
D010785	Photophosphorylation	The use of light to convert ADP to ATP without the concomitant reduction of dioxygen to water as occurs during OXIDATIVE PHOSPHORYLATION in MITOCHONDRIA.	Photosynthetic Phosphorylation,Phosphorylation, Photosynthetic,Phosphorylations, Photosynthetic,Photophosphorylations,Photosynthetic Phosphorylations
D002736	Chloroplasts	Plant cell inclusion bodies that contain the photosynthetic pigment CHLOROPHYLL, which is associated with the membrane of THYLAKOIDS. Chloroplasts occur in cells of leaves and young stems of plants. They are also found in some forms of PHYTOPLANKTON such as HAPTOPHYTA; DINOFLAGELLATES; DIATOMS; and CRYPTOPHYTA.	Chloroplast,Etioplasts,Etioplast
D004237	Diuron	A pre-emergent herbicide.	DCMU,3-(3,4-Dichlorophenyl)-1,1-dimethylurea
D006898	Hydroxylamines	Organic compounds that contain the (-NH2OH) radical.
D013058	Mass Spectrometry	An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.	Mass Spectroscopy,Spectrometry, Mass,Spectroscopy, Mass,Spectrum Analysis, Mass,Analysis, Mass Spectrum,Mass Spectrum Analysis,Analyses, Mass Spectrum,Mass Spectrum Analyses,Spectrum Analyses, Mass