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Heparin interferes with albumin determination by dye-binding methods. 1979

P Bonvicini, and G Ceriotti, and M Plebani, and G Volpe

Heparin, when added in vitro at rather high concentrations (400--1000 kilounits/L) as an anticoagulant, interferes with albumin determination by bromcresol green by forming an insoluble precipitate with this dye. The interference is much less with bromcresol purple. It can be abolished by addition of hexadimethrine bromide.

UI MeSH Term Description Entries
D001961 Bromcresol Green An indicator and reagent. It has been used in serum albumin determinations and as a pH indicator. Tetrabromocresolsulfonphthalein,Bromocresol Green,Green, Bromcresol,Green, Bromocresol
D006493 Heparin A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts. Heparinic Acid,alpha-Heparin,Heparin Sodium,Liquaemin,Sodium Heparin,Unfractionated Heparin,Heparin, Sodium,Heparin, Unfractionated,alpha Heparin
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001322 Autoanalysis Method of analyzing chemicals using automation. Autoanalyses
D012709 Serum Albumin A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules. Plasma Albumin,Albumin, Serum
D013053 Spectrophotometry The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.

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