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Conversion of single-stranded DNA of phage varphiX174 to the double-stranded replicative form in Escherichia coli uses enzymes essential for initiation and replication of the host chromosome. These enzymes can now be purified by the assay that this phage system provides. The varphiX174 conversion is distinct from that of M13. The reaction requires different host enzymes and is resistant to rifampicin and streptolydigin, inhibitors of RNA polymerase. However, RNA synthesis is essential for varphiX174 DNA synthesis: the reaction is inhibited by low concentrations of actinomycin D, all four ribonucleoside triphosphates are required, and an average of one phosphodiester bond links DNA to RNA in the isolated double-stranded circles. Thus, we presume that, as in the case of M13, synthesis of a short RNA chain primes the synthesis of a replicative form by DNA polymerase. Initiation of DNA synthesis by RNA priming is a mechanism of wide significance.
R Schekman, and
W Wickner, and
O Westergaard, and
D Brutlag, and
K Geider, and
L L Bertsch, and
A Kornberg
June 1976,
Proceedings of the National Academy of Sciences of the United States of America,
R Schekman, and
W Wickner, and
O Westergaard, and
D Brutlag, and
K Geider, and
L L Bertsch, and
A Kornberg
October 1977,
Proceedings of the National Academy of Sciences of the United States of America,
R Schekman, and
W Wickner, and
O Westergaard, and
D Brutlag, and
K Geider, and
L L Bertsch, and
A Kornberg
February 1972,
Journal of molecular biology,
R Schekman, and
W Wickner, and
O Westergaard, and
D Brutlag, and
K Geider, and
L L Bertsch, and
A Kornberg
July 1977,
Proceedings of the National Academy of Sciences of the United States of America,
R Schekman, and
W Wickner, and
O Westergaard, and
D Brutlag, and
K Geider, and
L L Bertsch, and
A Kornberg
July 1976,
Virology,
R Schekman, and
W Wickner, and
O Westergaard, and
D Brutlag, and
K Geider, and
L L Bertsch, and
A Kornberg
February 1969,
Journal of molecular biology,
R Schekman, and
W Wickner, and
O Westergaard, and
D Brutlag, and
K Geider, and
L L Bertsch, and
A Kornberg
March 1974,
Proceedings of the National Academy of Sciences of the United States of America,
R Schekman, and
W Wickner, and
O Westergaard, and
D Brutlag, and
K Geider, and
L L Bertsch, and
A Kornberg
August 1972,
Journal of molecular biology,
R Schekman, and
W Wickner, and
O Westergaard, and
D Brutlag, and
K Geider, and
L L Bertsch, and
A Kornberg
September 1975,
Journal of virology,
R Schekman, and
W Wickner, and
O Westergaard, and
D Brutlag, and
K Geider, and
L L Bertsch, and
A Kornberg
August 1972,
Journal of molecular biology,
