Biomaterial Database

Tetramethylbenzidine--a substitute for benzidine in hemoglobin analysis. 1979

R C Lijana, and M C Williams

Plasma hemoglobin is routinely measured by some modification of the benzidine-hydrogen peroxide method, particularly at low concentrations. Because benzidine is carcinogenic, and thus impractical for clinical use under current restrictions, the identification of a noncarcinogenic benzidine derivative has become important. 3,3',5,5'-tetramethylbenzidine fulfills this criterion, and consequently a method is described in this paper which uses it for hemoglobin determinations in the range of 1 to 100 mg/dl. Absorption spectra show this method to be far less influenced by plasma proteins and other substances than the benzidine method, and moreover the sensitivity of TMB is at least seven times that of benzidine under comparable conditions.

UI	MeSH Term	Description	Entries
D007202	Indicators and Reagents	Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)	Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D006454	Hemoglobins	The oxygen-carrying proteins of ERYTHROCYTES. They are found in all vertebrates and some invertebrates. The number of globin subunits in the hemoglobin quaternary structure differs between species. Structures range from monomeric to a variety of multimeric arrangements.	Eryhem,Ferrous Hemoglobin,Hemoglobin,Hemoglobin, Ferrous
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001560	Benzidines	Very toxic industrial chemicals. They are absorbed through the skin, causing lethal blood, bladder, liver, and kidney damage and are potent, broad-spectrum carcinogens in most species.	Bianilines,Biphenyldiamines
D013053	Spectrophotometry	The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
D013329	Structure-Activity Relationship	The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.	Relationship, Structure-Activity,Relationships, Structure-Activity,Structure Activity Relationship,Structure-Activity Relationships