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An in vitro system for perifusion of rat pancreatic islets has been utilized to define the effects of cholinergic agents on the dynamics of insulin release. In the absence of glucose the effects of either acetylcholine or acetyl-beta-methylcholine were minimal at concentrations up to 10(-5) mM. In the presence of low glucose concentration (2.4 mM), both of the muscarinic agents produced dose-dependent biphasic insulin release. Under these conditions significant insulin release was observed over both phases at concentrations of the muscarinic agents as low as 10(-8) mM. Further, the dose response curves relating muscarinic concentration to the total amount of insulin released in each of the two phases showed lack of parallelism between the curves. Nicotinic acid in concentrations up to 10(-5) mM had no effect on insulin release in the presence of 2.4 mM glucose. When the glucose concentration was increased to 16.4 mM, the effects of the muscarinic agents were significantly less than those observed in the presence of 2.4 mM glucose. This held true whether the effect was defined as absolute increment due to the muscarinic agent or as percentage of enhancement. Atropine inhibited insulin release induced by both acetylcholine and by 16.4 mM glucose. These data indicate that cholinergic stimulation can play a significant role in modifying insulin release patterns.
R Sharp, and
S Culbert, and
J Cook, and
A Jennings, and
I M Burr
January 1971,
European journal of clinical investigation,
R Sharp, and
S Culbert, and
J Cook, and
A Jennings, and
I M Burr
September 1971,
The American journal of physiology,
R Sharp, and
S Culbert, and
J Cook, and
A Jennings, and
I M Burr
November 1970,
The Journal of clinical investigation,
R Sharp, and
S Culbert, and
J Cook, and
A Jennings, and
I M Burr
February 1989,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
R Sharp, and
S Culbert, and
J Cook, and
A Jennings, and
I M Burr
January 1983,
Diabetes,
R Sharp, and
S Culbert, and
J Cook, and
A Jennings, and
I M Burr
December 1986,
Diabetes,
R Sharp, and
S Culbert, and
J Cook, and
A Jennings, and
I M Burr
February 1965,
Klinische Wochenschrift,
R Sharp, and
S Culbert, and
J Cook, and
A Jennings, and
I M Burr
December 1966,
Diabetologia,
R Sharp, and
S Culbert, and
J Cook, and
A Jennings, and
I M Burr
January 1986,
Advances in experimental medicine and biology,
R Sharp, and
S Culbert, and
J Cook, and
A Jennings, and
I M Burr
September 1979,
Experientia,
