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We have used the isolated perfused lung (IPL) preparation from the rat to determine whether uptake of choline from the vascular compartment could limit the rate of synthesis of phosphatidyl-choline (PC). The uptake of choline was rapid and did not saturate at a concentration of 10 mM. The rate of incorporation of choline into phospholipid was saturated above 0.1 mM choline. Whereas, uptake and incorporation were depressed at 4 C, uptake was neither dependent on the extracellular sodium concentration nor inhibited by equimolar concentrations of hemicholinium-3 (HC-3). We could find no evidence that uptake might limit synthesis of lung lecithin and conclude that uptake is either by free diffusion, or by a carrier-mediated process with a very high Km.
R G Johnson, and
M A Lugg, and
T E Nicholas
January 1970,
Canadian journal of physiology and pharmacology,
R G Johnson, and
M A Lugg, and
T E Nicholas
August 1980,
Proceedings of the National Academy of Sciences of the United States of America,
R G Johnson, and
M A Lugg, and
T E Nicholas
June 1989,
Journal of applied physiology (Bethesda, Md. : 1985),
R G Johnson, and
M A Lugg, and
T E Nicholas
April 1969,
Biochemical pharmacology,
R G Johnson, and
M A Lugg, and
T E Nicholas
December 1990,
Lipids,
R G Johnson, and
M A Lugg, and
T E Nicholas
October 1984,
Lipids,
R G Johnson, and
M A Lugg, and
T E Nicholas
October 1970,
Biochimica et biophysica acta,
R G Johnson, and
M A Lugg, and
T E Nicholas
August 1989,
Journal of neurochemistry,
R G Johnson, and
M A Lugg, and
T E Nicholas
May 1968,
Biochimica et biophysica acta,
R G Johnson, and
M A Lugg, and
T E Nicholas
March 1970,
Brain research,
