BIOMAT Database Logo BIOMAT Database Logo

Postribosomal complexes containing eukaryotic initiation factor eIF-2. 1979

H Amesz, and T Haubrich, and H O Voorma

Eukaryotic initiation factors are found in the post-ribosomal subunits. We have analyzed the factor activities from the supernatant by means of zonal centrifugation followed by Sepharose-heparin affinity chromatography. They exist both as free factors, sedimenting in a broad range from 4 to 7S, and complexed with other protein(s) with a sedimentation value of 16-20S. This complexed fraction contains besides eIF-2 another activity which exhibits a profound stimulation on amino acid incorporation in crude lysates and appears to counteract the heme-regulated inhibitor.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D010448 Peptide Initiation Factors Protein factors uniquely required during the initiation phase of protein synthesis in GENETIC TRANSLATION. Initiation Factors,Initiation Factor,Factors, Peptide Initiation,Initiation Factors, Peptide
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D012156 Reticulocytes Immature ERYTHROCYTES. In humans, these are ERYTHROID CELLS that have just undergone extrusion of their CELL NUCLEUS. They still contain some organelles that gradually decrease in number as the cells mature. RIBOSOMES are last to disappear. Certain staining techniques cause components of the ribosomes to precipitate into characteristic "reticulum" (not the same as the ENDOPLASMIC RETICULUM), hence the name reticulocytes. Reticulocyte
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D046911 Macromolecular Substances Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure. Macromolecular Complexes,Macromolecular Compounds,Macromolecular Compounds and Complexes,Complexes, Macromolecular,Compounds, Macromolecular,Substances, Macromolecular

Related Publications

H Amesz, and T Haubrich, and H O Voorma
Eukaryotic initiation factors eIF-2 and eIF-3: interactions, structure and localization in ribosomal initiation complexes.
January 1991, Biochimie,
H Amesz, and T Haubrich, and H O Voorma
Transfer-RNA, magnesium and the formation of ternary complexes by eukaryotic initiation factor eIF-2.
June 1989, Biochemistry international,
H Amesz, and T Haubrich, and H O Voorma
Therapeutic targeting of eukaryotic initiation factor (eIF) 4E.
February 2023, Biochemical Society transactions,
H Amesz, and T Haubrich, and H O Voorma
GTP interacts with the gamma-subunit of eukaryotic initiation factor eIF-2.
October 1984, FEBS letters,
H Amesz, and T Haubrich, and H O Voorma
Binding and release of eukaryotic initiation factor eIF-2 and GTP during protein synthesis initiation.
January 1978, Proceedings of the National Academy of Sciences of the United States of America,
H Amesz, and T Haubrich, and H O Voorma
Affinity labeling by a photoreactive GTP analogue of the delta-subunit of eukaryotic initiation factor eIF-2 in different initiation complexes.
March 1988, Biochemistry international,
H Amesz, and T Haubrich, and H O Voorma
The p46 subunit of eukaryotic initiation factor (eIF)-4F exchanges with eIF-4A.
March 1993, The Journal of biological chemistry,
H Amesz, and T Haubrich, and H O Voorma
Purification of eukaryotic initiation factors eIF-2, eIF-2B and eIF-2 alpha kinase from bovine liver.
August 1993, Preparative biochemistry,
H Amesz, and T Haubrich, and H O Voorma
Origins of robustness in translational control via eukaryotic translation initiation factor (eIF) 2.
May 2018, Journal of theoretical biology,
H Amesz, and T Haubrich, and H O Voorma
Dissociation of eukaryotic ribosomes by purified initiation factor EIF-3.
September 1973, Biochemical and biophysical research communications,
Copied contents to your clipboard!