-transparent.png){kind=logo}
The six fractions of rat liver DNA (DNAL) and hepatoma Zaidel DNA (DNAHZ) were obtained by thermal fractionation of these DNAs on a HAP column at equivalent temperature intervals. The percent of two thermolabile fractions obtained at 62--78 degrees is approximately 10% higher for DNAHZ than for DNAL. The reassociation kinetics were carried out till Cot = 5.10(-1). Computer analysis of the kinetics showed considerable difference between the parameters (percent of the sequence and rate constant) only for these thermolabile fractions DNAL and DNAHZ. The two-fold reduction of the reassociation rate for the second thermolabile fraction DNAHZ (63--78 degrees) can be associated with the presence of short uncomplementary pieces in the fast repeating sequences. On the other hand the increase of the rate constant for the initial part of the kinetics reassociation of the first thermolabile fraction (62--73 degrees) of DNAHZ is due to the nicks which are revealed by alkali sucrose centrifugation of the DNAHZ fractions.
A B Fonarev, and
A V Shugaliĭ, and
A L Tikhonravov, and
K P Khanson, and
I N Todorov
January 1974,
Methods in enzymology,
A B Fonarev, and
A V Shugaliĭ, and
A L Tikhonravov, and
K P Khanson, and
I N Todorov
January 1978,
Molekuliarnaia biologiia,
A B Fonarev, and
A V Shugaliĭ, and
A L Tikhonravov, and
K P Khanson, and
I N Todorov
January 1974,
Ukrains'kyi biokhimichnyi zhurnal,
A B Fonarev, and
A V Shugaliĭ, and
A L Tikhonravov, and
K P Khanson, and
I N Todorov
November 1974,
Biulleten' eksperimental'noi biologii i meditsiny,
A B Fonarev, and
A V Shugaliĭ, and
A L Tikhonravov, and
K P Khanson, and
I N Todorov
November 1975,
International journal of radiation biology and related studies in physics, chemistry, and medicine,
A B Fonarev, and
A V Shugaliĭ, and
A L Tikhonravov, and
K P Khanson, and
I N Todorov
March 1980,
Biokhimiia (Moscow, Russia),
A B Fonarev, and
A V Shugaliĭ, and
A L Tikhonravov, and
K P Khanson, and
I N Todorov
July 1973,
Biochemical genetics,
A B Fonarev, and
A V Shugaliĭ, and
A L Tikhonravov, and
K P Khanson, and
I N Todorov
January 1977,
Biochimica et biophysica acta,
A B Fonarev, and
A V Shugaliĭ, and
A L Tikhonravov, and
K P Khanson, and
I N Todorov
April 1989,
Biochemical genetics,
A B Fonarev, and
A V Shugaliĭ, and
A L Tikhonravov, and
K P Khanson, and
I N Todorov
April 1979,
Analytical biochemistry,
