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1. When WI-38 human diploid fibroblasts form confluent monolayers, DNA synthesis and cell division almost completely cease. A change of medium causes these density-inhibited cells to proliferate and within 1h after the application of the stimulus there is an increase in template activity of the chromatin isolated from stimulated cells. 2. The number of binding sites for Escherichia coli RNA polymerase was determined on chromatin from WI-38 cells by two different methods, i.e. incorporation of [(3)H]UTP into RNA in the absence of reinitiation, and incorporation of [gamma-(32)P]GTP into chain termini. 3. Both methods indicate that the capacity of chromatin to bind E. coli RNA polymerase is increased in WI-38 cells stimulated to proliferate. 4. The increase in the number of binding sites for E. coli RNA polymerase parallels the increase in chromatin template activity and suggests that the latter reflects an increase in the number of initiation sites, rather than an increase in the rate of transcription.
B T Hill, and
R Baserga
January 1975,
La Ricerca in clinica e in laboratorio,
B T Hill, and
R Baserga
August 1975,
Chemico-biological interactions,
B T Hill, and
R Baserga
September 1976,
Biochimica et biophysica acta,
B T Hill, and
R Baserga
August 1971,
Proceedings of the National Academy of Sciences of the United States of America,
B T Hill, and
R Baserga
June 1972,
The Journal of biological chemistry,
B T Hill, and
R Baserga
February 1974,
Experimental and molecular pathology,
B T Hill, and
R Baserga
February 1979,
Journal of cellular physiology,
B T Hill, and
R Baserga
May 1974,
Proceedings of the National Academy of Sciences of the United States of America,
B T Hill, and
R Baserga
March 1976,
Biochimica et biophysica acta,
B T Hill, and
R Baserga
January 1981,
Molecular and cellular biology,
