BIOMAT Database Logo BIOMAT Database Logo

Sensitivities of radioimmunoassay and enzyme-linked immunosorbent assay for detection of hepatitis B surface antigen. 1979

C A Hyland, and E C Mason, and P A Harden, and A E Shaw, and D Maurer, and S L Hope

Radioimmunoassay (RIA) using Austria II (Abbott) was found to be more sensitive for the detection of HBsAg than the Hepanostika Micro-Elisa system (Organon Teknika), by a factor of 5- to 20-fold for subtype 'ad' (with one exception), and 2- to 5-fold for subtype 'ay'. The sensitivities of RIA and the enzyme-linked immunosorbent assay (Elisa) have also been evaluated in the screening of 178 selected serum samples contributed by 7 different laboratories. Of these, 149 were found to be positive for HBsAg by RIA, 142 were positive by Elisa and 36 were positive by CEP.

UI MeSH Term Description Entries
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D001782 Blood Donors Individuals supplying blood or blood components for transfer to histocompatible recipients. Blood Donor,Donor, Blood,Donors, Blood
D003378 Counterimmunoelectrophoresis Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow. Electrosyneresis,Immunoelectroosmophoresis,Immunoelectrophoresis, Countercurrent,Immunoelectrophoresis, Crossover,Countercurrent Immunoelectrophoresis,Crossover Immunoelectrophoresis
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005069 Evaluation Studies as Topic Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D006514 Hepatitis B Surface Antigens Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen. Australia Antigen,HBsAg,Hepatitis B Surface Antigen,Antigen, Australia
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

C A Hyland, and E C Mason, and P A Harden, and A E Shaw, and D Maurer, and S L Hope
Enzyme-linked immunosorbent assay for hepatitis B surface antigen.
October 1977, The Journal of infectious diseases,
C A Hyland, and E C Mason, and P A Harden, and A E Shaw, and D Maurer, and S L Hope
An enzyme-linked immunosorbent-assay test for hepatitis B surface antigen.
August 1977, Journal of clinical pathology,
C A Hyland, and E C Mason, and P A Harden, and A E Shaw, and D Maurer, and S L Hope
Enzyme-linked immunosorbent assay for detection of antibody to the hepatitis B surface antigen-associated delta antigen.
August 1981, Journal of clinical microbiology,
C A Hyland, and E C Mason, and P A Harden, and A E Shaw, and D Maurer, and S L Hope
Development of enzyme-linked immunosorbent assay for hepatitis B e antigen.
March 1990, Journal of the Medical Association of Thailand = Chotmaihet thangphaet,
C A Hyland, and E C Mason, and P A Harden, and A E Shaw, and D Maurer, and S L Hope
Enzyme-linked immunosorbent assay for detection of receptors for polymerized human serum albumin on hepatitis B surface antigen particles.
October 1984, Journal of immunological methods,
C A Hyland, and E C Mason, and P A Harden, and A E Shaw, and D Maurer, and S L Hope
Enzyme-linked immunosorbent assay for detection of immunoglobulin M antibody to hepatitis B core antigen.
March 1981, Journal of clinical microbiology,
C A Hyland, and E C Mason, and P A Harden, and A E Shaw, and D Maurer, and S L Hope
[Enzyme-linked assay for detection of hepatitis B virus surface antigen (author's transl)].
December 1979, Zhonghua Minguo wei sheng wu xue za zhi = Chinese journal of microbiology,
C A Hyland, and E C Mason, and P A Harden, and A E Shaw, and D Maurer, and S L Hope
Experimental conditions affecting the sensitivity of enzyme-linked immunosorbent assay (ELISA) for detection of hepatitis B surface antigen (HBsAg).
January 1983, Bulletin of the World Health Organization,
C A Hyland, and E C Mason, and P A Harden, and A E Shaw, and D Maurer, and S L Hope
Development of a sensitive enzyme-linked immunosorbent assay for detection of hepatitis B surface antigen using novel monoclonal antibodies.
October 2010, Avicenna journal of medical biotechnology,
C A Hyland, and E C Mason, and P A Harden, and A E Shaw, and D Maurer, and S L Hope
Use of an enzyme-linked immunosorbent assay for screening hybridoma antibodies against hepatitis B surface antigen.
January 1982, Journal of immunological methods,
Copied contents to your clipboard!