-transparent.png){kind=logo}
A stable and predictable production system is described for pilot plant quantities (milligram) of human lymphoid interferon, using suspension culture of an African Burkitt's lymphoma derived cell line Namalva with induction by Newcastle disease virus, B-1 strain. Cell cultures were grown in impeller-driven 50-liter fermentors with dilution of the postinduction culture using serum-free medium. High levels of dissolved oxygen were necessary for optimum cell growth. A total of 4,207 liters of interferon culture was produced in a series of 116 fermentor runs. An average yield of 3.5 log(10) international units of interferon per ml was realized before processing. Trichloroacetic acid was used to precipitate the interferon. An average of 3.35 log(10) international units of interferon per ml was recovered in the final nonpurified product.
F Klein, and
R T Ricketts, and
W I Jones, and
I A DeArmon, and
M J Temple, and
K C Zoon, and
P J Bridgen
January 1986,
Methods in enzymology,
F Klein, and
R T Ricketts, and
W I Jones, and
I A DeArmon, and
M J Temple, and
K C Zoon, and
P J Bridgen
January 1981,
Methods in enzymology,
F Klein, and
R T Ricketts, and
W I Jones, and
I A DeArmon, and
M J Temple, and
K C Zoon, and
P J Bridgen
December 1980,
Cancer treatment reviews,
F Klein, and
R T Ricketts, and
W I Jones, and
I A DeArmon, and
M J Temple, and
K C Zoon, and
P J Bridgen
January 1983,
Rivista di biologia,
F Klein, and
R T Ricketts, and
W I Jones, and
I A DeArmon, and
M J Temple, and
K C Zoon, and
P J Bridgen
January 1980,
Journal of interferon research,
F Klein, and
R T Ricketts, and
W I Jones, and
I A DeArmon, and
M J Temple, and
K C Zoon, and
P J Bridgen
January 1981,
Acta microbiologica Academiae Scientiarum Hungaricae,
F Klein, and
R T Ricketts, and
W I Jones, and
I A DeArmon, and
M J Temple, and
K C Zoon, and
P J Bridgen
January 1986,
Methods in enzymology,
F Klein, and
R T Ricketts, and
W I Jones, and
I A DeArmon, and
M J Temple, and
K C Zoon, and
P J Bridgen
October 1977,
The Journal of biological chemistry,
F Klein, and
R T Ricketts, and
W I Jones, and
I A DeArmon, and
M J Temple, and
K C Zoon, and
P J Bridgen
January 1977,
Texas reports on biology and medicine,
F Klein, and
R T Ricketts, and
W I Jones, and
I A DeArmon, and
M J Temple, and
K C Zoon, and
P J Bridgen
January 1981,
Developments in biological standardization,
