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The bacteriocin produced by Mycobacterium smegmatis ATCC 14468 was isolated, and a study was made of its chemical, physical, and biological properties. No appreciable bacteriocin activity was found in the culture supernatant fluids, but it was released in appreciable quantities after disruption of the cells. The material was purified 49-fold by means of chromatography on diethylaminoethyl-cellulose, ammonium sulfate fractionation, gel filtration on Sephadex G-200, and chromatography on diethylaminoethyl-Sephadex A-50. Its molecular weight was determined to be approximately 75,000 from the elution profile on Sephadex G-200 chromatography. The bacteriocin was resistant to deoxyribonuclease, ribonuclease, lipase, ultraviolet irradiation, and freeze-thawing, whereas it was relatively less thermostable and was sensitive to proteolytic enzymes. The lethal effect of the bacteriocin was demonstrated by the decrease in viable counts of the bacteriocin-sensitive indicator strain, M. diernhoferi ATCC 19340. The bacteriocin preparation inhibited the growth of HeLa-S3 cells.
H Saito, and
T Watanabe, and
H Tomioka
January 1962,
Acta biochimica Polonica,
H Saito, and
T Watanabe, and
H Tomioka
April 2007,
The FEBS journal,
H Saito, and
T Watanabe, and
H Tomioka
March 1988,
Biochimica et biophysica acta,
H Saito, and
T Watanabe, and
H Tomioka
August 1983,
Archives of biochemistry and biophysics,
H Saito, and
T Watanabe, and
H Tomioka
May 1975,
Archives of biochemistry and biophysics,
H Saito, and
T Watanabe, and
H Tomioka
December 1971,
Biochimica et biophysica acta,
H Saito, and
T Watanabe, and
H Tomioka
January 1983,
Microbiology and immunology,
H Saito, and
T Watanabe, and
H Tomioka
May 1979,
Biochimica et biophysica acta,
H Saito, and
T Watanabe, and
H Tomioka
October 1975,
The Journal of biological chemistry,
H Saito, and
T Watanabe, and
H Tomioka
January 2003,
Methods in molecular biology (Clifton, N.J.),
