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A single-stranded specific nuclease has been purified from amyloryzine obtained from the mould fungi Aspergillus cryzae. The nuclease under study resembles the enzymes described in the literature in its ability to hydrolyze single-stranded nucleic acids. However, the enzyme essentially differs from previously known nucleases in some catalytic properties, particularly in its ability for degradation of poly A. It has been shown that the enzyme also hydrolyzes the synthetic dinucleotide pTpT to mononucleoside phosphates.
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