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Messenger RNA was purified from human foreskin fibroblasts FS11, a high interferon-producer line, after induction with synthetic double-stranded RNA. The mRNA was translated in a cell-free protein-synthesis system from rabbit reticulocytes. The translation products, containing biologically active human interferon, were immunoprecipitated by a serum from rabbits immunized against partially purified interferon. Analysis of the immunoprecipitate by polyacrylamide gel electrophoresis in dodecylsulfate shows that the product of human fibroblast interferon mRNA is a 23000-Mr polypeptide. Methods are described for the synthesis and rapid identification of this polypeptide, which should be useful for structural analysis of interferon and isolation of its mRNA.
J Weissenbach, and
M Zeevi, and
T Landau, and
M Revel
May 1978,
Biochemical and biophysical research communications,
J Weissenbach, and
M Zeevi, and
T Landau, and
M Revel
August 1976,
European journal of biochemistry,
J Weissenbach, and
M Zeevi, and
T Landau, and
M Revel
April 1987,
Biochemical and biophysical research communications,
J Weissenbach, and
M Zeevi, and
T Landau, and
M Revel
January 1983,
Methods in enzymology,
J Weissenbach, and
M Zeevi, and
T Landau, and
M Revel
February 1979,
European journal of biochemistry,
J Weissenbach, and
M Zeevi, and
T Landau, and
M Revel
May 1982,
Proceedings of the National Academy of Sciences of the United States of America,
J Weissenbach, and
M Zeevi, and
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M Revel
July 1985,
Virology,
J Weissenbach, and
M Zeevi, and
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M Revel
March 1991,
Biochimica et biophysica acta,
J Weissenbach, and
M Zeevi, and
T Landau, and
M Revel
January 1981,
Methods in enzymology,
J Weissenbach, and
M Zeevi, and
T Landau, and
M Revel
April 1987,
Nihon juigaku zasshi. The Japanese journal of veterinary science,
