-transparent.png){kind=logo}
A flow diagram for the automated determination of ferricyanide reductase activity in red blood cells was prepared in the modules from AutoAnalyzer AA I (Technicon Instruments Inc). Ferricyanide reductase assay can be substituted for assay of cytochrome b5 reductase (EC 1.6.2.2), which plays a major role in reducing methaemoglobin in erythrocytes, and is defective specifically in the erythrocytes of patients with hereditary methaemoglobinaemia. The effective sampling rate of the analysis is 30/h, and less than 0.05 ml of whole blood is required. Interference of haemoglobin with absorption by potassium ferricyanide at 420 nm is effectively exculded by dialysis. This automated method was compared with the accepted diaphorase method, and it distinguished clearly the ferricyanide reductase activity of cord bloods from that of adult bloods. The activity of the blood from a patient with hereditary methaemoglobinaemia was only residual. It is suggested that the method is useful as a mass screening test for hereditary methaemoglobinaemia.
K Tanishima, and
N Fukuda, and
M Takeshita, and
Y Takizawa, and
T Kitamura, and
Y Yoneyama
March 1981,
Clinical chemistry,
K Tanishima, and
N Fukuda, and
M Takeshita, and
Y Takizawa, and
T Kitamura, and
Y Yoneyama
January 2020,
The National medical journal of India,
K Tanishima, and
N Fukuda, and
M Takeshita, and
Y Takizawa, and
T Kitamura, and
Y Yoneyama
March 1981,
British journal of haematology,
K Tanishima, and
N Fukuda, and
M Takeshita, and
Y Takizawa, and
T Kitamura, and
Y Yoneyama
February 1963,
Acta medica Scandinavica,
K Tanishima, and
N Fukuda, and
M Takeshita, and
Y Takizawa, and
T Kitamura, and
Y Yoneyama
January 1990,
British journal of anaesthesia,
K Tanishima, and
N Fukuda, and
M Takeshita, and
Y Takizawa, and
T Kitamura, and
Y Yoneyama
May 1981,
The Biochemical journal,
K Tanishima, and
N Fukuda, and
M Takeshita, and
Y Takizawa, and
T Kitamura, and
Y Yoneyama
May 1978,
Scandinavian journal of haematology,
K Tanishima, and
N Fukuda, and
M Takeshita, and
Y Takizawa, and
T Kitamura, and
Y Yoneyama
April 1978,
Medical laboratory sciences,
K Tanishima, and
N Fukuda, and
M Takeshita, and
Y Takizawa, and
T Kitamura, and
Y Yoneyama
March 1980,
Analytical biochemistry,
K Tanishima, and
N Fukuda, and
M Takeshita, and
Y Takizawa, and
T Kitamura, and
Y Yoneyama
April 1971,
The Medical journal of Australia,
