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A highly sensitive radioimmunoassay for rat urinary kallikrein (minimal detectable amount, 40 pg/tube) has been developed. The assay uses a sheep antibody (Keq = 3.25 X 10(10)M-1) against purified Sprague-Dawley rat urinary kallikrein in a final dilution of 1 : 1,200,000. The assay incorporates a convenient and inexpensive PEG technique for separation of free from bound antigen. Parallel standard curves with rat urine or kidney homogenates were obtained. No cross-reactivity with human or dog urine samples or purified human urinary kallikrein was seen. Correlations among this assay, an esterolytic method, and a kininogenase radioimmunoassay for kallikrein were highly significant, with only the esterolytic assay demonstrating any significant nonspecificity. The radioimmunoassay can detect changes in urinary kallikrein levels produced by a maneuver known to alter urinary kallikrein excretion.
K Shimamoto, and
H S Margolius, and
J Chao, and
A R Crosswell
January 1979,
Advances in experimental medicine and biology,
K Shimamoto, and
H S Margolius, and
J Chao, and
A R Crosswell
October 1976,
Biochemical pharmacology,
K Shimamoto, and
H S Margolius, and
J Chao, and
A R Crosswell
January 1987,
Journal of immunoassay,
K Shimamoto, and
H S Margolius, and
J Chao, and
A R Crosswell
May 1988,
Biochemical pharmacology,
K Shimamoto, and
H S Margolius, and
J Chao, and
A R Crosswell
July 1980,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
K Shimamoto, and
H S Margolius, and
J Chao, and
A R Crosswell
January 1986,
Advances in experimental medicine and biology,
K Shimamoto, and
H S Margolius, and
J Chao, and
A R Crosswell
January 1987,
Archivos de biologia y medicina experimentales,
K Shimamoto, and
H S Margolius, and
J Chao, and
A R Crosswell
October 1978,
Horumon to rinsho. Clinical endocrinology,
K Shimamoto, and
H S Margolius, and
J Chao, and
A R Crosswell
October 1981,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
K Shimamoto, and
H S Margolius, and
J Chao, and
A R Crosswell
January 1978,
Klinische Wochenschrift,
